Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
网络转录分析 网络释义 1. 转录分析 又如进行中的转录分析(nuclear run on transcription assay)时,在体外将细胞核分离出来,然后在α -32P-ATP 的存 在下进行转 … www.docin.com|基于2个网页
Meng L, Lemaux PG (2003) A simple and rapid method for nuclear run-on transcription assays in plants. Plant Mol Biol Rep 21:65-71Meng L, Lemaux P (2003) A simple and rapid method for nuclear run-on transcription assays in plants . Plant Mol Biol Rep 21 : 65–71....
As evidenced by nuclear run-on transcription assays, both CT-1 and alpha-adrenergic stimulation lead to an increase in ANF gene transcription. Transient ... T Taga - 《Journal of Biological Chemistry》 被引量: 591发表: 1996年 Glucocorticoids induce transcription and expression of the alpha 1B ...
For the second batch, libraries were run on NovaSeq PE150 (Illumina, USA) to produce 6 GB of raw data per sample (20 M paired-end reads). Preparation of datasets Raw RNA-seq data comprised of 9 biological replicates per class (3D-MCF7, 3D-MCF10A, 2D-MCF7, 2D-MCF10A). Data from...
YAP-MAMLD1 concentrates transcription factors and co-activators, including BRD4, MED1 and TEAD, in condensates while excluding transcriptional repressive PRC2, and induces long-range enhancer–promoter interactions that promote transcription and oncogenic programmes. Blocking condensate-mediated transcriptional...
transcriptional control and transcription factors nuclear translocation protein–protein interaction INTRODUCTION The eukaryotic CCAAT-box binding nuclear factor Y (NF-Y) consists of three different subunits, A, B, and C. The three heterologous human NF-Y subunits and their homologous subunits in yeast...
Briefly, such fragments can be either synthesized (if no isotope labeling or introduction of ribose/base modifications is required) or prepared by in vitro run-off transcription (leaves 3′-OH) using co-transcriptional 5′- or 3′- cis- or trans- ribozyme cleavage (for 5′-OH or 3′-P,...
Two novel and related C2H2 zinc finger proteins that are highly expressed in the brain, CTIP1 and CTIP2 (COUPTF-interacting proteins1 and 2, respectively), were isolated and shown to interact with all members of the chicken ovalbumin upstream promoter transcription factor (COUP-TF) subfamily of...
(P = 0.006) and intronsP = 0.003 (Fig.3gand Supplementary Figs.1–3). No common NUMTs were within the 500 bp region flanking transcription start sites (TSS), consistent with selection against NUMTs that disrupt gene function (Fig.3hand Extended Data Fig.5d). Consistently, gene...