必应词典为您提供nuclear-run-on-assay的释义,网络释义: 核连缀分析;进行性核连缀转录分析;核转录活性测定;
Nuclei Run-on是一种用于检测细胞内正在转录的mRNA的技术。其原理是通过迅速冷冻细胞核，使正在转录的mRNA复合体处于冻结状态，随后加入ATP、GTP、CTP和32P-UTP，重新启动并终止mRNA的转录，从而标记冻结时正在转录的mRNA。提取mRNA后，通过cDNA探针和对照cDNA标记尼龙膜检测目标mRNA。细胞核的提取过程包括：...
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,核连缀实验, 答案 Northern印迹杂交(Northern blot).原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.DNA印迹技术由Southern于1975年创建,称为Southern印迹技术,RNA印迹技术...相关推荐...
The nuclear run-on assay is used to measure the transcriptional activity of selected endogenous genes. Nuclei are isolated from appropriate cells using techniques that keep engaged RNA polymerase complexes bound to genomic DNA. Subsequent incubation with the four ribonucleotide triphosphates, one of ...
NuclearRun-OnAssayStephenT.SmaleINTRODUCTIONThenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiationratecon..
Nuclei Run-on 原理：迅速冷冻，使细胞核内正在转录的mRNA复合体处于冻结状态 加入ATP/GTP/CTP/32P-UTP，重新恢复mRNA的转录并使之结束（标记冻结时正在转录的mRNA)，提取mRNA 以cDNA探针及对照cDNA标记尼龙膜后检测目标mRNA 2、操作过程：细胞核的提取 处理细胞（培养于10厘米培养皿）吸去培养液，迅速...
"nuclear run-on assay(=run-ontranscription assay)" Chinese translation ("nuclear run-on assay(=run-ontranscription assay)" in Chinese, "nuclear run-on assay(=run-ontranscription assay)" 中文翻译, "nuclear run-on assay(=run-ontranscription assay)"发音): ...
Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
White, J.D. and LaGamma, E.F., Neuropeptide gene transcription in central and peripheral nervous system tissues by nuclear run-on assay, Methods Enzymol., 168 (1989) 681-690.White JD , LaGamma EF . 1989 . Neuropeptide gene transcription in central and peripheral nervous system tissue by ...
Dilute resulted cDNA and use in qRT-PCR. 1. G. Patroneet al., Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR.Biotechniques29, 1012, 1016 (2000).Medline©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | ...