必应词典为您提供nuclear-run-on-assay的释义,网络释义: 核连缀分析;进行性核连缀转录分析;核转录活性测定;
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,核连缀实验, 答案 Northern印迹杂交(Northern blot).原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法.DNA印迹技术由Southern于1975年创建,称为Southern印迹技术,RNA印迹技术...相关推荐...
The nuclear run-on assay is used to measure the transcriptional activity of selected endogenous genes. Nuclei are isolated from appropriate cells using techniques that keep engaged RNA polymerase complexes bound to genomic DNA. Subsequent incubation with the four ribonucleotide triphosphates, one of ...
我想问一下关于nuclear run-on assay的原理以及其与Northern blot的比较,谢谢 网友 1 最佳答案 回答者:网友 Northern印迹杂交(Norther德阻节假长块n blot)。原理:将RNA固定于尼龙膜上以DNA探针识别待检mRNA这是一种将RNA从琼脂糖凝胶中转印到硝酸纤维素膜上的方法。DNA来自印迹技术由Southern于1975年创建,称360...
2.27M 文档页数: 7页 顶/踩数: 0/0 收藏人数: 0 评论次数: 0 文档热度: 文档分类: 待分类 系统标签: nuclearrunradiolabeledgenepolymerasenascent NuclearRun-OnAssayStephenT.SmaleINTRODUCTIONThenuclearrun-onassaywasdevelopedasamethodforestablishingthatthetranscriptioninitiationratecontributestotheregulatedexpression...
Nuclear run on transcription assay NUCLEAR RUN-ON TRANSCRIPTION Reagents:1.50X Denhardt’s solution: 1% Ficoll, 1% polyvinylpyrrolidone, and 1% bovine serum albumin (BSA). Filter sterilize.2.Dnase I 3.Hybridization buffer: 50% formamide, 6X SSC, 10X Denhardt’s solution, 0.2% SDS 4....
"nuclear run-on assay(=run-ontranscription assay)" Chinese translation ("nuclear run-on assay(=run-ontranscription assay)" in Chinese, "nuclear run-on assay(=run-ontranscription assay)" 中文翻译, "nuclear run-on assay(=run-ontranscription assay)"发音): ...
The stability of β2R mRNA was unchanged by Dex, and a nuclear run-on assay revealed that Dex approximately doubled the transcriptional rate of the β... JC Mak,M Nishikawa,PJ Barnes - 《Am J Physiol》 被引量: 644发表: 1995年 Effect of beta2-adrenergic receptor polymorphism on response...
Dilute resulted cDNA and use in qRT-PCR. 1. G. Patroneet al., Nuclear run-on assay using biotin labeling, magnetic bead capture and analysis by fluorescence-based RT-PCR.Biotechniques29, 1012, 1016 (2000).Medline©2022 Baidu |由 百度智能云 提供计算服务 | 使用百度前必读 | 文库协议 | ...
网络转录分析 网络释义 1. 转录分析 又如进行中的转录分析(nuclear run on transcription assay)时,在体外将细胞核分离出来,然后在α -32P-ATP 的存 在下进行转 … www.docin.com|基于2个网页