We examined the subcellular localization of the GFP-ACQOS protein and found that its nuclear translocation in response to osmotic stress was suppressed in aot19. We suggest that NUP85 is essential for the nuclear translocation of ACQOS, and the loss-offunction mutation of ...
7.9.2.4 Nuclear Pore Complex The nucleus exchanges information with the cytoplasm via NPCs, which are megadalton, multi-tiered protein structures that span the double bilayer of the nuclear envelope.38,39 In mammalian cells, NPCs are approximately 120 nm in diameter with a thickness of approximatel...
and an ancestral coatomer elements (ACE) domain. These protein folds are used in a modular arrangement to form subcomplexes in the NPC scaffold rings [19]. A key component of the outer rings is the Y-complex, also known as the Nup107-Nup160 ...
To prove this hypothesis, we performed RNAi-mediated knockdown screening of several nuclear pore complex proteins. This led to the identification of nucleoporin-85 (nup85) as indispensable for HIV replication in macrophages. To eliminate siRNA non- specific events as contributory to the observed ...
In Xenopus laevis, full-length Nup160 consists of a seven-bladed β-propeller followed by an extended α-helical domain, and its C-terminal fragment is located at the vertex of the Y complex and sandwiched between Sec13 homolog 1 (Seh1) and SEC13-like protein 1 (Sec13) (Zhu et al. ...
In a genetic screen for nucleoporin-interacting components, a novel nuclear pore protein Nup84p, which exhibits homology to mammalian Nup107p, was isolated. Nup84p forms a complex with five proteins, of which Nup120p, Nup85p, Sec13p, and a Sec13p homolog were identified. Upon isolation ...
Mechanism of mRNA Transport Through the Nuclear Pore Nuclear export of mRNA is an essential process in eukaryotic gene expression. Immediately after initiation of transcription, mRNA binds to various proteins to form an mRNA–protein complex (messenger ribonucleoprotein: mRNP) [35,36]. The mRNA precu...
Figure 2. The Nuclear Pore Is a Dense Phase of Disordered Proteins That Forms a Selective Barrier. (A) Schematic of the nuclear pore complex. The nuclear pore is lined with disordered domains of nucleoporin (Nup) proteins containing phenylalanine and glycine (FG) dyad repeats. Image reproduced...
In a screen for mutants defective in nucleocytoplasmic export of mRNA, we have identified a new component of the Saccharomyces cerevisiae nuclear pore complex (NPC). The RAT9/NUP85 (ribonucleic acid trafficking) gene encodes an 84.9-kDa protein that we have localized to NPCs by tagging the RAT...
The Nuclear Pore Complex (NPC) has emerged as an important hub for processing various types of DNA damage. Here, we uncover that fusing a DNA binding domain to the NPC basket protein Nup1 reduces telomere relocalization to nuclear pores early after telom