Functional analysis of nuclear pore complex protein Nup62 ⁄ p62 using monoclonal antibodies. Hybridoma (Larchmt) 2006; 25; 51-59.Fukuhara T, Sakaguchi N, Katahira J, Yoneda Y, Ogino K, Tachibana T. Functional analysis of nuclear pore complex pro- tein Nup62/p62 using monoclonal antibodies...
This work demonstrates a 1:1:1 stoichiometry of the NUP62 complex, but non-canonical stoichiometries when a coiled-coil partner is absent. Article PubMed PubMed Central Google Scholar Amlacher, S. et al. Insight into structure and assembly of the nuclear pore complex by utilizing the ...
Another large protein–membrane assembly at the subcellular scale is thenuclear pore complex(NPC)[94•,95], located in the double bilayers of thenuclear envelopemediating particle exchange between nucleus and cytoplasm. Nsp1, anuclear poreprotein, is the major component of NPC.MDsimulations, perfo...
accumulates at the perinuclear cellular compartment and co-localizes with the nuclear pore complex Nup62 protein, suggesting a role in nuclear membrane ... D Bertozzi,R Iurlaro,O Sordet,... - 《Taylor & Francis》 被引量: 39发表: 0年 The nuclear pore complex prevents sister chromatid recomb...
and exit. The nuclear pore complex is the largest protein complex in the cell, with a mass of about 65 MDa in yeast and about double that in higher eukaryotes. It consists of about 30 different proteins (known as nucleoporins or nups). The pore complex has 8-fold symmetry (Fig. ...
Nup62 to form a heterotrimeric hub [12,26]. The Nup214-Nup88 subcomplex facilitates nuclear export by directly binding to the nuclear export receptor Crm1 [67]. The FG domain in the extreme C terminal of Nup214 also interacts with the mRNA export protein Nxf1 [68]. Nup42 interacts ...
(polyQ) expansion in the N-terminal of the Huntingtin protein [52]. An interesting proteomics study in 2001 discovered that many NPC proteins, particularly Nup62, co-aggregate with polyQ aggregates [53]. These results initiated a hypothesis in which HD mutations may confer toxicity through ...
2b, 3a) as well as lamin B1, a protein found close to the inner nuclear membrane. Fig. 3: “Side view” of the NPCs with Nup96, Nup62 and Pom121 imaged by splitSMLM. a Scheme of the NPCs at the NE. ONM, outer nuclear membrane; INM, inner nuclear membrane. b Averaged “side...
The nuclear pore complex (NPC) is a giant protein assembly that penetrates the double layers of the nuclear membrane. The overall structure of the NPC has approximately eightfold symmetry and is formed by approximately 30 nucleoporins. The great size and complexity of the NPC have hindered the ...
We found that the karyopherin beta-related export receptor CRM1 competes with the Tap transport domain for binding to Nup214 but not for binding to Nup62 or Nup153, suggesting that the Tap and CRM1 nuclear export pathways converge at the cytoplasmic periphery of the nuclear pore complex. ...