Here, we use a genetic approach to further characterize the molecular and cellular architecture of the sex-peptide response in Drosophila females.Screening for mutations that affect the sensitivity to sex-peptide, we identified the channel nuclear pore protein Nup54 gene as an essential component for...
7.9.2.4 Nuclear Pore Complex The nucleus exchanges information with the cytoplasm via NPCs, which are megadalton, multi-tiered protein structures that span the double bilayer of the nuclear envelope.38,39 In mammalian cells, NPCs are approximately 120 nm in diameter with a thickness of approximatel...
The post-mating response induced by male-derived sex-peptide in Drosophila females is a well-established model to elucidate how complex innate behaviors are hard-wired into the brain. Here, we found that the channel nuclear pore protein Nup54 is essential for the sex-peptide response as viable...
and an ancestral coatomer elements (ACE) domain. These protein folds are used in a modular arrangement to form subcomplexes in the NPC scaffold rings [19]. A key component of the outer rings is the Y-complex, also known as the Nup107-Nup160 ...
In Xenopus laevis, full-length Nup160 consists of a seven-bladed β-propeller followed by an extended α-helical domain, and its C-terminal fragment is located at the vertex of the Y complex and sandwiched between Sec13 homolog 1 (Seh1) and SEC13-like protein 1 (Sec13) (Zhu et al. ...
Two multiprotein complexes, the 10-member Y complex (named because of its shape) and the five-member Nup93 complex make up the scaffold of the pore. The cytoplasmic and nuclear rings are assembled from 16 copies each of the Y complex. The Nup93 complex forms the framework of the spoke ...
The nuclear basket attaches to the nucleoplasmic side of the nuclear pore complex (NPC), coupling transcription to mRNA quality control and export. The basket expands the functional repertoire of a subset of NPCs in Saccharomyces cerevisiae by drawing a
The nuclear pore complex protein Nup88 is overexpressed in tumor cells. Cancer Res 1999; 59: 5408–11. Google Scholar Udeani GO, Gerhauser C, Thomas CF, Moon RC, Kosmeder JW, Kinghorn AD, et al. Cancer chemopreventive activity mediated by deguelin, a naturally occurring rotenoid. Cancer...
Yeast cells expressing nuclear pore protein Nup159-Atg8-split Venus (red) and fiducial beads (red, intense signal, round, 1 µm) are visible. Together with the information of the grid foil quality, suitable positions for FIB Milling can be determined. The green...
Mechanism of mRNA Transport Through the Nuclear Pore Nuclear export of mRNA is an essential process in eukaryotic gene expression. Immediately after initiation of transcription, mRNA binds to various proteins to form an mRNA–protein complex (messenger ribonucleoprotein: mRNP) [35,36]. The mRNA precu...