The nuclear pore complex (NPC) is a macromolecular protein assembly embedded in the double lipid bilayer of the nuclear membrane, and is the sole gateway of macromolecular traffic between the nucleus and cytoplasm. The NPC serves as a barrier to inhibit the free molecular flux in and out of ...
Illustrations of the nuclear pore complex and classical nucleocytoplasmic transport.AStructure of the nuclear pore complex showing the main subcomplexes and their component nucleoporins (Nups).Created with BioRender.com.BIn classical nuclear import, protein cargo containing the nuclear localization signal ...
This arrangement creates a central channel ∼120 nm wide and ∼85 nm deep called the nuclear pore, which serves as a protein-dense passageway for transport of cargo between the nucleus and the cytoplasm (Figure 2A). Small molecules can freely diffuse through the pore but larger molecules (...
Mechanism of mRNA Transport Through the Nuclear Pore Nuclear export of mRNA is an essential process in eukaryotic gene expression. Immediately after initiation of transcription, mRNA binds to various proteins to form an mRNA–protein complex (messenger ribonucleoprotein: mRNP) [35,36]. The mRNA precu...
The nuclear pore complex (NPC) is a massive structure comprised of 33 known protein components organized into five main structural domains–the cytoplasmic filaments (yellow), the inner and outer nuclear rings (green), the transmembrane components (purple), the nuclear core components (blue), and...
nupvesicle coatsThe nuclear pore complex (NPC) is one of the largest protein machines in the cell and forms the sole conduit for nucleocytoplasmic transport in eukaryotes. The NPC is composed of an eightfold radially symmetric scaffold of architectural proteins that anchor a set of phenylalanine...
Thus, forks arrested by a DNA-bound protein complex transiently relocate to the NP in S-phase cells. To examine if the dynamics of the active RFB changes with NP enrichment, we monitored the mobility of the GFP-LacI focus by single-particle tracking (SPT) in living cells (Supplementary Fig...
Nup210 depletion suppresses lung metastasis in mouse models of breast cancer. Mechanistically, NUP210 interacts with LINC complex protein SUN2 which connects the nucleus to the cytoskeleton. In addition, the NUP210/SUN2 complex interacts with chromatin via the short isoform of BRD4 and histone H...
In Xenopus laevis, full-length Nup160 consists of a seven-bladed β-propeller followed by an extended α-helical domain, and its C-terminal fragment is located at the vertex of the Y complex and sandwiched between Sec13 homolog 1 (Seh1) and SEC13-like protein 1 (Sec13) (Zhu et al. ...
The molecular mechanisms that allow HIV to integrate into particular sites of the host genome are poorly understood. Here we tested if the nuclear pore complex (NPC) facilitates the targeting of HIV integration by acting on chromatin topology. We show th