Six-week-old female BALB/c mice were used for the lung metastasis experiments. MDA-MB-231 cells (1 × 106) in 200 μl of PBS solution were injected into the lateral tail vein of nude mice. After intraperitoneal injection of luciferase substrate and isoflurane inhalation anesthesia, bio...
Subareolar versus peritumoral injection for location of the sentinel lymph node. Ann Surg. 1999;229:860–5. Article CAS Google Scholar Krag DN, Anderson SJ, Julian TB, Brown AM, Harlow SP, Ashikaga T, et al. Technical outcomes of sentinel-lymph-node resection and conventional axillary-...
Prior to injection, a 2 μl aliquot was kept aside and measured to know the exact activity loaded on the column. During the first 30 minutes the flow was set to 0.1 mL/min to remove free metal and impurities or side-products. The eluted fraction was then used to measure ...
The efflux of nuclear radioactivity was slowest for MDA-MB-468 cells. Cytotoxicity was correlated with EGFR expression. Uptake was greater in MDA-MB-468 than in MCF-7 xenografts and improved with preinjection of a 100-fold excess of unlabeled DTPA-hEGF. Nuclear importation was higher in liver...
We have shown that COX-2 is essential for the growth of MDA-MB-231 cells in the fat pad of SCID mice and for their extrapulmonary colonization following injection in the tail vein of SCID mice. The molecular changes that follow shRNA-mediated silencing of COX-2 include a significant ...
Injection areas were gently massaged for 2-3 min to generate the flow of dye along the lymphatics. The first 1-4 lymph nodes stained blue were designated SLN(s). The SLN specimen was then placed directly into formalin. Two designated pathologists performed routine pathologic evaluation 24-72 ...
Bekerman C, Pavel DG, Bitran J, Ryo UY, Pinsky S. The effects of inadvertent administration of antineoplastic agents prior to Ga-67 injection: concise communication.J Nucl Med1984;25: 430–435. Google Scholar Goldberg EM. Mediastinoscopy in assessment of lung cancer. In: Strauss MJ, ed.Lun...
the status of ER and PR along with HER2 has established a straightforward stratification for breast cancer which correlates with both survival outcomes and the selection of targeted therapeutic approaches [120,121]. Another study has demonstrated the overexpression of LRH1 in breast cancer cells playin...
We next performed intrathoracic co-injection of Padi4KO recipient cells with isolated NEPs or apoptotic debris (apoDBs) as a control. Tumor lesions were monitored for early metastatic outgrowth (Fig. 4c). As expected, co-injection with NEPs enhanced Padi4KO metastatic outgrowth at 72 h and ...
JMDA-MB-231-RR cells transfected with indicated shRNAs were injected into nude mice and then treated with irradiation 1 week after cell injection.ITumor volumes were measured every week (n = 5 for each group). *P < 0.05 by one-way analysis of variance followed by Tukey’s post-...