1、实时荧光定量PCR检测NPMl一mu认mRNA表达量的方法构建:采用相 对定量法,应用TaqMan探针技术,以管家基因ABL为内参,同时设置正常对照 和无模板对照;提取待测标本总RNA,测定其核酸浓度,进行10倍系列稀释后作 为反应模板,设定其拷贝数为101、102、103、104、105、106构建标准曲线;反应 ...
Mouse Npm1 qPCR Primer Pair,即小鼠Npm1 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
Human NPM1 qPCR Primer Pair,即人NPM1 qPCR引物对,主要用于基于SYBR Green的qPCR、One-Step qRT-PCR或semi-quantitative PCR。本引物为预先设计、经过qPCR验证、预混的引物对。 qPCR (Quantitative PCR)即定量PCR,也称实时荧光定量PCR或实时定量PCR (Real-time quantitative PCR)、实时PCR (Real-time PCR),是一...
一般情况下RQ-PCR检测NPM1的敏感性高于FCM,但也有部分患者出现NPM1-FCM+,可能与NPM1探针针对特殊类型突变的敏感性、疾病过程中的克隆演变以及标本的取材等原因有关。而此时的FCM+对复发的预测也有意义。 综上,在AML化疗过程中监测MRD,FCM+或NPM1+均对复发...
Methods NPM1 mutation(including A,B,D mutation type) was detected in 206 patients with newly diagnosed AML by real-time quantitative RT-PCR. Results The incidence of NPM1 mutation was 15.5% in total AML patients and 32.5% in normal karyotypes AML patients.The characteristics of 174 NPM1 ...
After being washed three times with low-salt wash buffer and one time with high-salt wash buffer, immunoprecipitated chromatin DNA was eluted and quantified using real-time quantitative PCR. Sequences of primers for ChIP Q-PCR were listed in Table S4. Statistical analysis Statistical tests and ...
Assessment of measurable residual disease (MRD) by real time quantitative polymerase chain reaction (RT-qPCR) forNPM1mutant transcripts [11] can be combined with the ELN risk stratification to inform therapeutic decisions, e.g. helping to select patients who may benefit from allogeneic haematopoietic...
for Sanger sequencing to verify mutant allele expression. Real-time PCR was utilized to examine relative expression ofDnmt3ainDnmt3afl-R878H/+Mx1-cre+animals prior to pIpC injection (primer sequences in TableS1). To verify cellular localization of Npm1cA, spleens fromNpm1cA/+R26FlpoERor ...
(includingA,B,D mutationtype)was detectedin206patientswith newlydiagnosedAML byreal—timequantitativeRT—PCR.Results Theinci— denceofNPMImutationwasl5.5% intotalAMLpatientsand32.5% innormalkaryotypesAML patients. Thecharacteristicsofl74NPM1wildtypepatientsv.s.thatof32NPM 1mutationpatientswasasfollow.me—...
(17例),低危(10例)三组,采用Real-time PCR SYBRGreen法分别检测三组与中HO-1与内参基因GAPDH m RNA转录本的相对表达量,与对照组比较.294例正常核型患者采用PCR产物直接测序法检测(FLT3-ITD,NPM1,DNMT3A,CEBPa)基因的突变情况,按NPM1+/FLT3-... 章亚明,王季石,韦四喜,... - 全国血液肿瘤学术大会暨全国...