(MRD) an informative clinical target.METHODS:Herein, we have developed a straightforward unique molecular identifier (UMI)-based amplicon next-generation sequencing method for the detection of NPM1-mutated MRD that addresses some of the limitations present in other assays.RESULTS:The NPM1 assay ...
Abstract Objective：To establish the droplet digital PCR (ddPCR) assay for the detection of NPM1type A mutation in patients with acute myeloid leukemia (AML) , and to evaluate its specificity, sensitivity and its value in clinical application. Methods：NPM1mutant and wild-type plasmids were used...
detectionofMRD. Methods 1.The expression level ofNPM1-mutAmRNA by real time quantitative polymerase chain reaction(RQ-PCR)assay:TheTaqmanprobe WASchosen for real—time PCRdetectionofNPM1-mutA,Thehouse-keepinggene ABLservedasa 4 contr01.Standardcurveswereconstructed ...
Interestingly, the bacterial two-hybrid assay revealed that the complement factor C1qB interacts with the Cap protein (Timmusk et al., 2006), the ligand of gC1qR. One is tempted to speculate, whether a ternary complex between Cap, C1qB and gC1qR is formed. Par-4 was first identified as...
ITD). Our patient had good outcome after CHT alone, suggesting that cases with exon 11 mutations may behave similarly to those with exon 12 mutations, but confirmatory studies are needed. MRD monitoring of these very rareNPM1mutations may require designing a patient-specific RT-qPCR assay [73]...
1基因DNA序列设计的,针对NPM1基因第12外显子突变热点设计了两条野生型探针,一条位于突变热点上,一条位于突变热点外,当突变热点存在插入,替换,缺失等突变时,位于突变热点的野生型探针则不能与模板紧密结合,所以该试剂盒仅用两对引物探针即可检出NPM1基因第12外显子突变热点的多种突变,并且其灵敏度高,可用于MRD...
Due to their frequency and stability, NPM1 mutations may become a new tool not only for risk stratification of AML patients at time of diagnosis, but also for monitoring minimal residual disease (MRD) during and after the course of therapy in AML with normal karyotype [14]. The use of ...
(CR1)NPM1-specific molecular MRD (n = 16) data. The total CD3-positive T-cell percentages correlated positively between FC and MIF (r = 0.53,p = 0.05), but were significantly lower by MIF (1.62% vs. 3.4%,p = 0.009). The percentage of mutant NPM1-positive (NPM1...
Flow cytometry, PCR, and NGS can all be employed for MRD detection, but each carries its own limitations. Though technically challenging, NGS has the advantage being able to simultaneously assay multiple mutations, thus providing for broader applicability. NPM1 is the most prevalently mutated gene...
We performed NPM1 MRD detection in blood collected from patients with AML during first CR (CR1) prior to alloHCT on a subset of patients from the Pre-MEASURE study (PMID: 36881031) using a highly-sensitive, commercially available DNA-based NPM1 NGS MRD assay. Methods: Of the 531 patients...