bindDNAnon-specifically.Filterbindingexperimentswith proteolyticfragmentsofMuArevealthattheN-terminal26 aminoacidsofdomainIIIbindssupercoiledDNAnon- specifically(3)andthatdomainIIcaninteractwithlinear non-specificDNA(4).TheTn3Tnpbindsnon-specificlinear restrictionfragments(5,6).Finally, many Tnps, ...
Non-specific binding When an antibody binds to unintended proteins (with highly similar epitopes) we speak of non-specificity. However, binding to closely related proteins may sometimes be unavoidable. Then the actual binding of the antibody may be specific, yet the antibody is deemed non-specific...
Blocking Non-specific Binding with Serum.A. CD14 was detected in paraffin-embedded human tonsil tissue using anti-human CD14 biotinylated affinity-purified polyclonal antibody (Catalog # BAF383). Tissue was subjected to antigen retrieval and stained using high sensitivity streptavidin conjugated to HRP ...
Introduction Ultrafiltration (UF) is used to separate unbound drugs; however, non-specific binding (NSB) may be a limiting factor of this technique. Pretreatment of UF devices has been suggested to reduce NSB. Therefore, the pretreatment methodologies for UF devices were evaluated in order to ...
Solving the Challenge of Non-Specific Binding | Waters Scientists Talk MaxPeak Premier Principal Research Scientist Mike Donegan discusses the challenge of non-specific binding of analytes and how Waters developed a system to allow researchers to improve...
For three clinical-stage antibodies with suboptimal combinations of off-target binding and self-association, the classifiers predicted variable-region mutations that optimized non-affinity interactions while maintaining high-affinity antibody-antigen interactions. Interpretable machine-learning models may ...
This signal solely originates from non-specific binding. To confirm the absence of DENV, DENV specific MAB's have been re-admitted at t = 2450 s. Since this does not lead to any change in the instrumental output signal, we thus have clear indication that no detectable viral material was ...
The current protocols for blocking background staining in immunohistochemistry are based on conflicting reports. Background staining is thought to occur as a result of either non-specific antibody (Ab) binding to endogenous Fc receptors (FcRs) or a combi
ATR-FT-IR spectroscopy was employed to study the kinetics of transport and binding within thin silica sol−gel films. Studies of transport of several non... Dion,Rivera,Joel,... - 《Analytical Chemistry》 被引量: 74发表: 2001年 Measurement of Peptide Binding to MHC Class II Molecules by...
976 Sequence-specific and Non-specific Rci Binding to sfxs segments, with segment A exhibiting the highest affinity to the Rci protein. The shufflon recombination sites have been defined as 19 bp repeat sequences based on their ...