Non-specific binding When an antibody binds to unintended proteins (with highly similar epitopes) we speak of non-specificity. However, binding to closely related proteins may sometimes be unavoidable. Then the actual binding of the antibody may be specific, yet the antibody is deemed non-specific...
The main principles of the present invention is the use of competitive antagonism by adding RuBisCo large subunit expression in vitro in immunoblotting experiments to eliminate or weaken western blot during deep background or non-specific hybridization, so that results can more realistically reflect ...
Concurrent with LPS administration, anti-histone H4 antibody (anti-H4) or non-specific IgG was administered to study the role of extracellular histones. ... Y Zhang,L Guan,J Yu,... - 《Respiratory Research》 被引量: 14发表: 2016年 Characterisation of cellular and humoral autoimmune responses...
Non-specific binding sites were blocked by 3% BSA. Tyrosine phosphorylation was initiated by adding the purified EGFR (10 µg for each sample) in 100 µL of assay buffer (20 mM Tris, pH 7.4; 10 mM MgCl2; 1 mM MnCl2; 0.2 mM ATP; 1 mM dithiothretiol; 25 mM...
(Millipore, USA). Non-specific binding was blocked on the PVDF membranes with PBS buffer containing 0.1% Tween-20, 2% BSA, and 5% nonfat dry milk. Blots were then incubated with anti-rabbit primary antibodies overnight at 4 °C. The next day, membranes were extensively washed, and then...
3D). The specific binding between MATR3 protein and MIDEAS-AS1 was further confirmed by RNA pull down following Western blot (Fig. 3E). Then, we performed RNA immunoprotein (RIP) assay using flag antibodies and revealed that MIDEAS-AS1 was significantly enriched in MATR3 immunoprecipitations ...
(ELISA), western blot (WB), flow cytometry, immunofluorescence, and focus reduction neutralization test (FRNT). Most of all, to our knowledge, we first provided a NP-specific mAb for the detection of SARS-CoV-2 variant live viruses including Alpha, Beta, Delta, and Omicron variants, which ...
specific tumor sites. This approach ensures the targeting effect and biosafety of the delivered drug in vivo. It is worth mentioning that the use of messenger RNA (mRNA) for vaccination, protein replacement therapy and cancer immunotherapy holds the potential to revolutionize the treatment of a ...
Several drugs and antibodies are in different stages of clinical trials for their efficacy in controlling liver fibrosis, while no specific treatment option is still available46. In this context, our study provides compelling evidence for the reduction in NAFLD activity score, liver fibrosis, ...
All mice were kept under specific temperature (18–22 °C), relative humidity (40–70%), and noise (<50 dB) conditions, with ad libitum access to water and food in an animal room with a 12-h light/dark cycle. The mice were randomly assigned to a normal group, an I/R group ...