Non-specific binding sites were blocked by 3% BSA. Tyrosine phosphorylation was initiated by adding the purified EGFR (10 µg for each sample) in 100 µL of assay buffer (20 mM Tris, pH 7.4; 10 mM MgCl2; 1 mM MnCl2; 0.2 mM ATP; 1 mM dithiothretiol; 25 mM...
giving rise to a fibrotic state, which can lead to hepatocellular carcinoma. Several drugs and antibodies are in different stages of clinical trials for their efficacy in controlling liver fibrosis, while no specific treatment option is still available...
Because the degradation products in the body are amino acids, which do not easily accumulate in specific tissues and organs, the toxic reaction is relatively weak. The binding interaction with the target protein could be strong and selective, ensuring the minimal non target adverse reactions38,70,...
Recently, urine perilipin-2 (PLIN-2) was demonstrated to be a sensitive and specific biomarker for the noninvasive, early detection of RCC and an indispensable indicator to distinguish cancer from a benign renal mass. However, current Western blot or ELISA PLIN-2 assays are complicated, ...
(ELISA), western blot (WB), flow cytometry, immunofluorescence, and focus reduction neutralization test (FRNT). Most of all, to our knowledge, we first provided a NP-specific mAb for the detection of SARS-CoV-2 variant live viruses including Alpha, Beta, Delta, and Omicron variants, which ...
To further examine specific differences in host response to each strain, an Analysis of Variance (ANOVA) was performed and genes found to be significant in the cell survival, stress response, and apoptosis were compared between different strain challenges under identical conditions (Tables 1, 2, 3...
the enrichment of p53 binding was measured by qRT-PCR using specific primers targeting the p53 REs in steroid hormones binding factors loci. In the precipitated DNA of HepG2 Nutlin-3a-treated cells, there was an enrichment for the p53 REs entailed in all steroid hormones binding factors and ...
binding properties [2]. Across the entire genome, YY1 binds to both gene regulatory elements and RNA species transcribed from these elements which was postulated to act as positive-feedback for YY1 binding to these regions [3]. Its specific function depends on its localization and is determined...
6d). Next, we performed EMSAs using nuclear extracts and DNA probes containing the predicted YY1 binding sites. Figure 6e shows that when DNA probes were incubated with nuclear extracts from A549 cells, a specific DNA–protein complex was observed (Fig. 6e, lane 2), and this complex was ...
Next, we hypothesised that long-chain ceramide synthesis in response to lipotoxicity was directly regulated by specific UPR pathways. Intracellular ceramide 40:1 and 42:1 concentrations were analysed in C2C12 myotubes co-treated with 200 µM palmitate or the BSA vehicle, and either 10 µ...