Non-reducing SDS-PAGE and western blot analysis of gp140 expression following co-transfection of multiple env plasmids.Emma, J. BowlesTorben, SchiffnerMaximillian, RosarioGemma, A. NeedhamMeghna, RamaswamyJoanna, McGouranBenedikt, KesslerCelia, LaBranche...
Vascular endothelial growth factor (VEGF) is a key regulator of NSCLC development via inhibition of immune cell differentiation, thereby reducing infiltration and promoting immune tumor cell escape [15].Cordyceps sinensis, the content of the Bailing capsule, has anti-tumor, enhanced immunity, resistanc...
reducing the inhibitory effect of miR-2110 onSp1; in turn, the elevatedSp1bound to the AFAP1-AS1 promoter region and activated its transcription, forming a positive feedback system. Certainly, these hypotheses need further exploration and discovery in follow-up research. In summary, we detected a...
Real-time PCR, flow cytometry, immunofluorescence and Western blot were used to evaluate the level of lncRNA H19, apoptosis, autophagy and some related proteins. The modified multiple ligase reaction was used to analyze the gene polymorphism of six SNPs in H19, rs217727, rs2067051, rs2251...
Integrin β3 (ITB3) is a subunit of the periostin receptor26. We generated A549 or NCI-H520 cells transfected withITB3small hairpin (sh) RNA, constitutively reducingITB3expression (Supplementary Fig.5a,b). To more specifically confirm the effect of periostin on NSCLC cells, we examined the ...
Long non-coding RNA heart and neural crest derivatives expressed 2-antisense RNA 1 overexpression inhibits the proliferation of cancer cells by reducing RU... Long non-coding RNA heart and neural crest derivatives expressed 2-antisense RNA 1 overexpression inhibits the proliferation of cancer cells ...
Some studies have indicated that intestine-specific agonists could alleviate fatty liver disease by altering BAs, reducing hepatic glucose production, and inducing thermogenesis in white adipose tissue without activating hepatic FXR.31,32 Conversely, inhibiting the intestinal FXR-ceramide axis has been ...
Previously we observed that VNUT-dependent ATP release and activation of purinergic signaling aggravates glucose metabolism by reducing insulin sensitivity in the liver [15,16], providing direct evidence that purinergic signaling is involved in glucose homeostasis. We wonder if VNUT is responsible for ...
In the present, the main approaches for measuring ncRNAs expression can be divided into two types.23One method with low throughput is represented by quantitative real-time polymerase chain reaction (RT-qPCR), northern blot, and in situ hybridization. Among these, RT-qPCR is due to its inexpensi...
Western blot (WB) WB was used to measure levels of proteins. Supernatant of the homogenized tissue was diluted with reducing buffer, Laemmli sample buffer (Biorad, 161–0737) and electrophoresed on Tris-HCL 4–20% pre-cast linear gradient gel (Biorad, 4561093) and transferred to PVDF membr...