具有降低的非特异性活性的pcr反应混合物 Having reduced non-specific activity of the reaction mixture pcr本发明提供了用于提高核酸扩增的特异性的方法,所述方法包括:将核酸分子与聚合酶Sso7DNA结合结构域缀合物和精氨酸,亚精胺或精胺一起温育. The present invention provides a method for increasing the ...
较普通PCR体系提高了2个数量级.此方法较其他方法操作简单成本低廉,且所得的热启动Taq DNA聚合酶稳定性好,便于保存,其灵敏性与特异性也更高.这种通过化学修饰形成的复合物达到热启动PCR目的的方法的建立,具有着重要的意义,是提高PCR灵敏度和特异性的重要方法之一.%Anhydride is capable of binding to and formin...
兔IgG (Fc specific, Heavy Chain) 单克隆抗体Rabbit IgG (Fc specific, Heavy Chain) Monoclonal Antibody700/30ul#1540/100ul#2520/200ul 公司简介 上海联祖生物科技有限公司 Shanghai lianzu Biotechnology Co. , Ltd.成立于2020年,坐落于中国魔都上海,提供生物试剂、免疫试剂、生化试剂、实验仪器及耗材、制药工...
single-cell suspension was obtained and staining was performed in four separate panels. Fresh tumour and non-adjacent lung single cell suspensions were pre-incubated with Fc receptor blocking solution (Biolegend) to reduce non-specific binding and subsequently stained according to the specific protocols...
For each sample, RNA was retro-transcribed into cDNA by using the GoScript™ Reverse Transcription System (Promega) and the expression of the target gene was assessed through Real-Time PCR with the GoTaq® qPCR Master Mix. To this purpose, target-specific mostly intron-spanning primers (...
As its name implies, plant lipid transfer protein (LTPs) were termed because of their functions that transfer phospholipids and fatty acids between membranesin vitro[1]. They were also named non-specific LTPs (nsLTPs) due to the character of non-specific binding to different lipids. Plant nsLT...
In questo scenario, quando si esegue msinfo32 per controllare la configurazione di PCR7, viene visualizzato come Binding not possible.In this scenario, when you run msinfo32 to check the PCR7 Configuration, it's displayed as Binding not possible....
congolense-specific and T. theileri-specific bands. Also in the nested ITS PCR, amplification of T. vivax-specific sequences is enhanced by adding Q-solution, while for T. congolense and T. theileri amplification is reduced. Table 5. Number of positives among 23 TvPRAC PCR positive and ...
‘pixel-to-tile’ map would instead correspond to a layer of phosphorylation or binding circuitry that activates or deactivates specific components on the basis of the levels of upstream information-bearing molecular signals. Within artificial cells47, multicomponent nucleation may be an especially ...
et al. Differential strengths of molecular determinants guide environment specific mutational fates. PLoS Genet. 14, e1007419 (2018). Article PubMed PubMed Central Google Scholar Chen, P. & Zhang, J. Antagonistic pleiotropy conceals molecular adaptations in changing environments. Nat. Ecol. Evol...