NK-92MI[NK92MI] CELL<人恶性非霍奇金淋巴瘤患者的自然杀伤细胞系> 更多供应商 上海弘顺生物科技有限公司 联系电话: 15901914505 15901914505 产品介绍: 中文名称:NK-92MI[NK92MI] Cell<人恶性非霍奇金淋巴瘤患者的自然杀伤细胞系> 英文名称:NK-92MI[NK92MI] Cell ...
NK-92MI[NK92MI] Cell;人恶性非霍奇金淋巴瘤患者的自然杀伤细胞 细胞产品包装:复苏形式:T25培养瓶(一瓶)或冻存形式:1ml冻存管(两支) JCA-1细胞系;细胞传代方法:1:2-1:3传代;每周换液2-3次;细胞形态特性:详见细胞说明书;细胞生长特性:贴壁或悬浮,详见细胞说明书部分;细胞背景资料:详见相关文献介绍 ...
悬浮细胞的生长形态一般分为单个生长和聚团生长，今天来分享两个聚团生长的细胞中，比较难养，需要注意培养技巧的细胞，分别是NK92和NK92MIcell culture 1培养条件：cell culture NK-92 75%MEMα＋0.2mM Inositol＋0.1mM β-mercaptoethanol＋0.02mM Folic Acid＋12.5% HS＋12.5% FBS+0.1mM β-Mer+200...
悬浮细胞的生长形态一般分为单个生长和聚团生长,今天来分享两个聚团生长的细胞中,比较难养,需要注意培养技巧的细胞,分别是NK92和NK92MI cell culture 1.培养条件: NK-92 75%MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+12.5% HS+12.5% FBS+0.1mM β-Mer+200U/ml IL-2 NK-92MI 7...
生长特性suspension, multicell aggregates 培养基MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+12.5% HS+12.5% FBS+1% P/S 形态特征lymphoblast 传代方法1:2-1:3 培养条件Atmosphere: Air, 95%; CO2, 5%。Temperature: 37℃
NF449 inhibits Gαs, blunts SP-induced expression of perforin and granzyme B, and represents a potential therapeutic avenue for reducing NK-cell mediated cytotoxicity.doi:10.1016/j.npep.2013.12.001Diandong, HouKefeng, SunWeixin, FuMoran, Wang...
cell culture 1.培养条件: NK-92 75%MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+12.5% HS+12.5% FBS+0.1mM β-Mer+200U/ml IL-2 NK-92MI 75%MEMα+0.2mM Inositol+0.1mM β-mercaptoethanol+0.02mM Folic Acid+12.5% HS+12.5% FBS+0.1mM β-Mer ...
NF449 inhibits Gαs, blunts SP-induced expression of perforin and granzyme B, and represents a potential therapeutic avenue for reducing NK-cell mediated cytotoxicity. 展开 关键词: NK92-MI cells Substance P Gs Gi Cytotoxicity Immunoregulation ...
关于NK细胞培养的问题,不能只认为培养基不好或试剂盒不好,很多客户在培养过程中遇到接种密度的问题、补液程序的问题、血浆问题以及样本的问题,所以影响NK细胞培养效果的因素非常之多,那么在细胞培养不太满意的情况下,我们应该按以下原则进行排查。 1、血液样本问题首先确认样本的状态,理论上来讲外周血样本应在抽取的6...
NK92-MI cellsInterferon gamma(IFN-g)Programmed cell death-ligand 1(PD-L1)Background and aims:Berberine is one of the most promising clinically tested natural alkaloids,and immunotherapy using natural killer(NK)cells is a potentially effective treatment for hepatocellular carcinoma(HCC).This study ...