Automated, high-throughput quantification of EGFP-expressing neutrophils in zebrafish by machine learning and a highly-parallelized microscopedoi:10.1371/journal.pone.0295711MACHINE learningBRACHYDANIOLARVAENEUTROPHILSOSTEICHTHYESTHRESHOLDING algorithmsNATURAL immunityMICROSCOPES...
NETQUANT: automated quantification of neutrophil extracellular traps. Front Immunol. 8, 1999 (2017). PubMed Google Scholar Azzouz, D., Khan, M. A., Sweezey, N. & Palaniyar, N. Two-in-one: UV radiation simultaneously induces apoptosis and NETosis. Cell Death Discov. 4, 51 (2018). ...
Automated flow cytometry enables high performance point-of-care analysis of leukocyte phenotypes. J Immunol Methods. 2019;474. https://doi.org/10.1016/j.jim.2019.112646. Leliefeld PHC, Pillay J, Vrisekoop N, Heeres M, Tak T, Kox M, et al. Differential antibacterial control by neutrophil ...
Interestingly, both in humans during acute inflammation [182] and in cancer patients [81], a neutrophil phenotype with relatively high expression of PD-L1 was observed. In the acute inflammation model, this subset presented an IFN-induced transcription profile. Indeed, high IFN-γ concentrations co...
What is a neutrophil automated count? What can low neutrophils mean? How many neutrophils are in blood? What causes high lymphocytes and low neutrophils? How are neutrophils involved in the body's defense against pathogens? What causes low WBC and low neutrophils? What attracts neutrophils? What...
The cell number and purity of neutrophils were determined using an automated haematology analyser (KX-21N, Sysmex, Japan) and only samples with more than 90% purity were used for experiments. After isolation, neutrophils were rested for 20 min and followed by processing for further experiments. ...
A normal Neutrophils Count isbetween 2,500 and 7,000. The process of measuring the Absolute Neutrophil Count is automated by the analyzer and shows in some CBCs as the neutrophil automated count. Neutrophilia is diagnosed when the CBC shows an Absolute Neutrophil Count over 7,000. ...
Donated blood was separated using the Revos® automated blood processing system (TerumoBCT, Japan), and buffy coats were used for neutrophil isolation. Neutrophils were isolated from fresh blood or from buffy coats at room temperature under sterile conditions using a class II biological safety ...
Densitometry was done using the automated digitizing software (Image J, NIH, Bethesda, USA). All phosphorylation levels were normalized to the protein expression of β-actin. Statistical analysis Between-group differences were examined for statistical significance using Student’s t-test. P-values of...
The cell number and purity of neutrophils were determined using an automated haematology analyser (KX-21N, Sysmex, Japan) and only samples with more than 90% purity were used for experiments. After isolation, neutrophils were rested for 20 min and followed by processing for further experiments...