immunosuppressive prophylactic treatment, and concomitant drug administration.45On the other hand, neutrophils isolated from human peripheral blood or mouse BM for in vitro studies are, according to the literature, not always purified at very high levels (<97%); they are therefore...
cell suspension was passed through 70- and 40-μm cell strainers. After RBC lysis, cells were washed and resuspended in staining buffer (1 × PBS/2% FBS). Total and trypan blue-negative (viable) cells were counted with an automated cell counter (BioRad). Representative images of propidium...
The derived parameters can be used to generate histograms, scattergrams, and/or other graphical displays in which the EGC and neutrophil populations are separated sufficiently such that automated enumeration of the respective populations can be achieved. FIG. 5 illustrates a neutrophil population502that ...
These data support the expression of the high-affinity receptor for IgG to be a characteristic feature of PMN during G-CSF therapy. Comparison of different CD64 antibodies. Fc receptors are members of the Ig-supergene family consisting of a large number of highly homologous molecules.31 To test...
Here, we report a haptotaxis assay, based on the classic under-agarose assay, which combines an optical patterning technique to generate surface-bound formyl peptide gradients as well as an automated imaging and analysis of a large number of migration trajectories. We show that human neutrophils ...
(α-CR1 and α-CR3). While CR3-blocking antibodies did not affect phagocytosis of C3b-E. coli, we observed that CR1-blocking antibodies potently reduced phagocytosis (Fig.1E). Inhibiting CR1 reduced both the absolute number of bacteria associated with neutrophils (Fig.1E) as well as the ...
(ΔhHv1), engineered by shortening the N-terminus and removing the C-terminus coiled-coil domain, as described5. Supplementary Figure12shows that while ΔhHv1 channels were operational, they did not respond to 75 µM Alb. We suspect our observation that high Alb concentrations were able...