immunosuppressive prophylactic treatment, and concomitant drug administration.45On the other hand, neutrophils isolated from human peripheral blood or mouse BM for in vitro studies are, according to the literature, not always purified at very high levels (<97%); they are therefore...
The cell number and purity of neutrophils were determined using an automated haematology analyser (KX-21N, Sysmex, Japan) and only samples with more than 90% purity were used for experiments. After isolation, neutrophils were rested for 20 min and followed by processing for further experiments...
Total leukocyte, neutrophil, lymphocyte, monocyte, eosinophil, and basophil were determined from K3EDTA anti-coagulated blood (6 mL) using an automated haematology analyzer (ADVIA 2120, Bayer Healthcare, Siemens, UK) equipped with software for bovine blood. Leukocyte immunostaining Acid citrate dextros...
However, automated analyzers cannot directly measure immature forms of neutrophils. A manual white blood cell count (WBC) differential is performed only when a specimen is flagged by the instrument as a result of high or low cell counts. This study was designed to determine how often significant...
Here, we report a haptotaxis assay, based on the classic under-agarose assay, which combines an optical patterning technique to generate surface-bound formyl peptide gradients as well as an automated imaging and analysis of a large number of migration trajectories. We show that human neutrophils ...
Complete blood count was done on LH750 automated hematology analyser (Beckman Coulter). VCS data; mean neutrophil volume (MNV), mean neutrophil conductivity (MNC) and mean neutrophil scatter (MNS) for all patients was recorded. MNV was high ( p <.0001) while MNS was lower ( p <.0001) ...
(SD) of 4 independent experiments. Statistical analysis was done using one-way ANOVA with a Dunnett correction for multiple comparisons and displayed only when significant compared to control “0 µg/mL C3b-DBCO” as * (p ≤ 0.05) and ** (p ≤ 0.01). (G) CFU count of ...
Imaging is done using a Nikon Ti-E inverted microscope controlled by MATLAB via Micromanager, allowing simultaneous automated imaging of multiple wells in groups. An environmental chamber was used to maintain a 37 °C temperature, and wells were imaged at 4X magnification every 30 s using ...
a,bmIHC images show the simultaneous detection of DAPI (blue), SAA (red), CD15+neutrophils (yellow), CD68+macrophages (brilliant blue), CD8+T cells (white), and PD-L1 (green) in human HCC peritumor TMA. The count of infiltrating immune cells between SAA high and SAA low samples is ...
18.The analyzer of claim 16, wherein the analyzer is configured to:a) apply one or more reliability criteria to:i) the neutrophil count, wherein the analyzer is configured to present the neutrophil count in the interface reporting results of the first test as the labeled neutrophil count; an...