Mature, low-density, immunosuppressive neutrophils with protective effects against aGVHD have also been described in mouse models. Accordingly, initial data showed that the low-density fraction obtained by density gradient centrifugation of splenocytes from G-CSF-treated mice contains a population of m...
The cell number and purity of neutrophils were determined using an automated haematology analyser (KX-21N, Sysmex, Japan) and only samples with more than 90% purity were used for experiments. After isolation, neutrophils were rested for 20 min and followed by processing for further experiments...
Aliquots of ovine whole blood and isolated neutrophils were taken and an automated full blood count and white blood cell differential was performed using the Abbott Cell-Dyn 3200 Multiparameter Haematology Analyser (Abbott Diagnostics Division, North Ryde, Australia). Purity was assessed based upon the...
However, automated analyzers cannot directly measure immature forms of neutrophils. A manual white blood cell count (WBC) differential is performed only when a specimen is flagged by the instrument as a result of high or low cell counts. This study was designed to determine how often significant...
Here, we report a haptotaxis assay, based on the classic under-agarose assay, which combines an optical patterning technique to generate surface-bound formyl peptide gradients as well as an automated imaging and analysis of a large number of migration trajectories. We show that human neutrophils ...
Imaging is done using a Nikon Ti-E inverted microscope controlled by MATLAB via Micromanager, allowing simultaneous automated imaging of multiple wells in groups. An environmental chamber was used to maintain a 37 °C temperature, and wells were imaged at 4X magnification every 30 s using ...
a,bmIHC images show the simultaneous detection of DAPI (blue), SAA (red), CD15+neutrophils (yellow), CD68+macrophages (brilliant blue), CD8+T cells (white), and PD-L1 (green) in human HCC peritumor TMA. The count of infiltrating immune cells between SAA high and SAA low samples is ...
Fibrinogen was measured by using the ACL TOP 500 (Instrumentation Laboratory, Bedford, MA, USA), a fully automated random-access multiparameter coagulation analyser, equipped with a photo-optical clot-detection unit. For HGB, mean platelet volume, RBCs and platelet count, EDTA was used as ...
Here, we answer to the conundrum; because the uterus contains ~500 µM Alb, the Vthresholdfor hHv1 shifts to ~ −30 mV (Supplementary Fig.10). Whereas Alb concentration is too low in semen to activate hHv1 prematurely, the level is high enough in the female reproductive tract to...
10.The method of claim 5, wherein the first test is performed using an analyzer configured to:a) apply one or more reliability criteria to:i) a neutrophil count, wherein the analyzer is configured to present the neutrophil count in the interface reporting results of the first test as the ...