confirming that neutrophils entirely depend on glycolysis. In agreement with this concept, bothOpa1N∆and controlLyz2Cre/Creneutrophils exhibit very low OCR (Supplementary Fig.9b, left panel). The limited OCR in neutrophils is even further reduced inOpa1N∆neutrophils which, however, is an ob...
(red). Arrows indicate decondensed nonaggregated NETs, arrowheads indicate diffuse NETs with decondensed nuclei and * indicates neutrophils with intact nuclei.cNETs were quantified using Fiji and expressed as percentage of NETs, percentage of staining under NETs per field of view and total area ...
Percentage inhibition of DC CD86 (Ai; flow cytometry) and IL-12 expression (Aii; ELISA) by MPO was assessed. (Bi) CD86 expression by DCs cultured with LPS/H2O2 in the presence of Cl alone or Cl + SNP (NO donor). (Bii) DCs were cultured with LPS/H2O2 and mouse MPO (0.125 μg/...
role-of-manual-immature-to-total-neutrophil-it-ratio-and-automated-immature-granulocyte-count-igcand-percentage-ig-in-thChandima Kulathilake
Blood counts were obtained using an Abacus automated counter (Diatron; Holliston, USA). Protocols for parabiosis experiments, long-term reconstitution assays, flow cytometry, homing assays, immunofluorescence experiments, intravital imaging, chemokine quantification, and other methods as well as additional...
aureus, the expression of CD44 by the milk neutrophils and the percentage of apoptotic B cells were lower in the milk cells from BLV-infected dairy cows, particularly those from animals with persistent lymphocytosis (PL). The lymphocyte subsets were not different among the groups, with the ...
The percentage of each immune cell subset (CD3, CD15, and CD68) was calculated by dividing the absolute number of each cell subset by the total number of infiltrating immune cells. To quantify immunofluorescence, a standard fluorescence-field scanning protocol was developed. Proportions of cells ...
Blood specimens were collected as part of physical examinations, and WBCs were measured in the MECs. The WBC parameters were determined using the Beckman Coulter method of counting and sizing, and the automated differential was based on cell volume, conductivity, and light scatter technology19. We...
g, Automated quantification (percentage of positively stained cells of total cells detected in inflamed areas) of IHC stainings shown in f. Each staining was quantified on inflamed tissue sections with low (n = 15), intermediate (n = 13) and high (n = 12) M4/M5 whole-...
rather simplistic and procrustean classification. Our results emphasize a possible role of NETs and neutrophil-derived mediators also in allergic asthmatics. These findings could suggest that neutrophils could play a pathogenic role not only in T2-low but also in a percentage of T2-high asthmatics...