To alter neuronal activity within the mixed-species co-culture system we incubated the cells overnight in the voltage-gated Na þ channel blocker tetrodotoxin (TTX), to block action potential (AP) firing. We then washed out the TTX in the presence of the GABAA (g-aminobutyric acid) ...
AAV vector production and purification were performed by Virovek (Hayward, California, USA) as previously described [31]. Briefly, Sf9 insect cells were cultured at 28 °C to ~107 cells/mL in Sf-900 II serum-free medium containing 100 U/mL of penicillin and 100 μg/mL streptomyci...
Latham JR, Pathirathna S, Jagodic MM, Choe WJ, Levin ME, Nelson MT, Lee WY, Krishnan K, Covey DF, Todorovic SM, Jevtovic-Todorovic V (2009) Selective T-type calcium channel blockade alleviates hyperalgesia in ob/ob mice. Diabetes 58:2656–2665 CASPubMed CentralPubMedGoogle Scholar Ledeen...
Chemicals, Peptides, and Recombinant Proteins polyethyleneimine “MAX” Polysciences, Inc. Cat. #24765 Dynasore Abcam Cat. #ab120192 (S)-3,5-DHPG Tocris Bioscience Cat. #0805 cycloheximide Sigma-Aldrich Cat. #C7698-1G RNase A Omega Bio-tek Cat. #D6942-02 RNase A Thermo Fisher Scientific ...
(d) Calcium video imaging performed on HEK-293 cells transiently transfected with either GluN1-1b WT, K178V mutated GluN1-1b or K190V mutated GluN1-1b in combination with WT GluN2A. Before an incubation with different treatment, two first NMDA stimulations (100 μM) were performed ...
200 μm. Quantification of CD31 positive cells performed in Control and CLN3Q352XECs. Mean fluorescence intensity of the CD31 channel normalized to the nuclear (Hoechst positive) signal was calculated for 12 random fields per condition using ImageJ (NIH) (Welch’s test; ns, not significant)...
Clones with and without m.3243A > G heteroplasmy showed positive expression of the pluripotency markers OCT4, NANOG, SOX2, and LIN28 (Figures S1A and S1B) and normal karyotypes (Figure S1C). We included a curated healthy iPSC line (control [CTR]; 409-B, Kyoto University; Kondo et al...
KIIIA and another small μ-conotoxin, SIIIA, have been shown to have analgesic properties in mouse inflammatory pain assays [101–103]. These μ-conotoxins have a framework that could be used to develop new analgesic drugs. Their small size, high affinity and unique VGSC channel selectivity ...
the Brain of Freely Behaving Wild Animals Kaoru Ide and Susumu Takahashi * Laboratory of Cognitive and Behavioral Neuroscience, Graduate School of Brain Science, Doshisha University, Kyotanabe 610-0394, Kyoto, Japan * Correspondence: stakahas@mail.doshisha.ac.jp Citation: Ide, K.; Takahashi, S. ...
必要に応じて、(:AB培地で100倍希釈で例えば青ニッスル)25分間蛍光ニッスルを持つセクションにラベルを付けます。 、AB培地を調製0.2 Mリン酸緩衝液250mlを混合する(PB; 51 mMのKH 2 PO 4、150mMののNa 2 HPO 4)、15 mlの5 M NaClを、15 mlの10%のTriton-X、220ミリリットルのddH...