Publication » PO27-TH-15 Frequency of false positive neuronal voltage-gated potassium channel antibody detection in serological evaluation for neurological autoimmunity.doi:10.1016/S0022-510X(09)71185-2Apiwattanakul, M.McKeon, A.Lennon, V.
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Activation curves were fitted by one Boltzmann distribution: G/Gmax = 1/{1 + exp[(V50 − V)/s]}, where V50 is the voltage at which the current is half-activated, and s is the slope factor. For single channel analysis, patches with a maximum of three active channels were used for...
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After MOM block, sections were incubated in primary antibody at 4 °C overnight after being diluted to optimal concentrations (anti-APOE 1:200 dilution (Cell Signaling); anti-calmodulin 1:100 dilution (Thermo Fisher); anti-CD68 1:100 dilution (Bio-Rad); anti-Cre recombinase 1:800 ...
Further, labeling of all niche cells with an antibody against the G1-phase marker MCM2-7 (except when they are progressing through S to M phase), suggests that these are not terminally differentiated cells as one would expect if some niche cells are serving in a support role for other, ...
7B) Immunostaining of occasional motor neuron perikaryons with monoclonal antibodies anti- NF axonal, 7C) Immunostaining of a large grain with anti-synaptophysin monoclonal antibody, 7D-7E) Immunostaining with anti-GFAP showing a significant astrogliosis in the anterior gray horns (7D) and in the ...
thus suggest that GABAARs and KCC2 may be both regulated by TrkB signaling. We therefore tested whether blocking TrkB in PNI animals could restore the original GABAAR subunit composition. Incubating spinal slices from PNI animals with a function-blocking anti-TrkB antibody49,57caused an inverse ...
Apolipoprotein E4 (APOE4) is the strongest known genetic risk factor for late-onset Alzheimer’s disease (AD). Conditions of stress or injury induce APOE expression within neurons, but the role of neuronal APOE4 in AD pathogenesis is still unclear. Here