To alter neuronal activity within the mixed-species co-culture system we incubated the cells overnight in the voltage-gated Na þ channel blocker tetrodotoxin (TTX), to block action potential (AP) firing. We then washed out the TTX in the presence of the GABAA (g-aminobutyric acid) ...
Guo Y, Chen J, Ji W, Xu L, Xie Y, He S, Lai C, Hou K, Li Z, Chen G, Wu Z. High-titer AAV disrupts cerebrovascular integrity and induces lymphocyte infiltration in adult mouse brain. Mol Therapy - Methods Clin Dev. 2023;31:101102. https://doi.org/10.1016/j.omtm.2023.08.021...
The lysates were precleared with Sepharose protein G beads (Protein G Sepharose 4 Fast Flow, GE Healthcare) before incubation with capture antibody (4 mg ml À 1) (monoclonal a-FLAG M2, F1804-50UG, Sigma, its isotype control mouse IgG1, k, 554121, BD Pharmingen, and a-FoxA1, ab...
Rabbit polyclonal anti pan-Nrxn Sclip and Südhof109 Clone G394; RRID: AB_2800397 Rabbit polyclonal anti RIM1/2 Acuna et al.110 Clone U952 Rabbit polyclonal anti Stx1a/b Zhou et al.111 Clone 438B Rabbit polyclonal anti PSD95 Patzke et al.71 Clone L667; RRID:AB_2800396 Mouse monoclona...
(G-ANT) and normalized cell viability to that in the other halves of the bisected slices that were exposed only to α-synM. The relative degree of LDTFof cell survival associated with α-synMin the presence of G-ANT was significantly lower than in absence of blockers of receptors of ...
Bologna LL, Pasquale V, Garofalo M, Gandolfo M, Baljon PL, Maccione A, Martinoia S, Chiappalone M (2010) Investigating neuronal activity by SPYCODE multi-channel data analyzer. Neural Netw 23:685–697 Article PubMed Google Scholar Carulli D, Broersen R, de Winter F, Muir EM, Meskovi...
The KH-type splicing regulatory protein (KHSRP) is an RNA-binding protein linked to decay of mRNAs with AU-rich elements. KHSRP was previously shown to destabilize Gap43 mRNA and decrease neurite growth in cultured embryonic neurons. Here, we have tested
3 MR selective antagonist canrenoate-K reversed the enhanced expression of pain signaling molecules TRPV1 (a), CGRP (b), Nav1.8 (c), and trkA (d) mRNA in nociceptive DRG neurons with inflammatory pain. a–d Chronic i.t. canrenoate-K administration to rats with Freund's complete ...
(Supplementary Fig.3b), which was accompanied by a reduction in all egg deposition motor elements (Supplementary Fig.3c–g). Abdominal contortions and the exploration programmes showed little or no differences with the respective controls (Supplementary Fig.3h–k), suggesting a possible compensation ...
In Parkinson’s disease (PD), Lewy pathology deposits in the cerebral cortex, but how the pathology disrupts cortical circuit integrity and function remains poorly understood. To begin to address this question, we injected α-synuclein (αSyn) preformed