Complementation of an Escherichia coli glycolysis mutant by Giardia lamblia triosephosphate isomerase. The Giardia lamblia triosephosphate isomerase (TIM) gene was cloned using a probe generated by a polymerase chain reaction that employed primers complement... MR Mowatt,EC Weinbach,TC Howard,... - ...
BioNetSim: A Petri net-based modeling tool for simulations of biochemical processes. Protein and Cell 3:225-229. (2012). J Gao,L Li,X Wu,... - 蛋白质与细胞:英文版 被引量: 0发表: 2012年 Biochemical Reaction Pathway Analysis Using Petri Net A systematic procedure has been developed to ...
Quantitative polymerase chain reaction To assess Piezo1 expression, RNA was extracted using the RNeasy Mini Kit (QIAGEN). The purity of RNA was then assessed using a NanoDrop (Thermo Fisher Scientific), and complementary DNA (cDNA) was synthesized with the SuperScript IV VILO Master Mix kit (In...
To demonstrate that Petri nets are indeed suitable for simulating metabolic pathways, the glycolysis and citric acid cycle are selected as well-understood examples of enzymatic reaction chains (metabolic pathways). The paper discusses the steps that lead from gaining necessary knowledge about the ...
While there is some theoretical support for a rate-affinity trade-off for particular reaction schemes12,13, this depends on untestable assumptions about the free energy profile and it does not apply to typical reaction schemes of membrane transport processes, such as facilitated diffusion. We will...
Reaction between the carbonyl group of reducing sugar and the amino group of proteins and lipids via a Schiff base reaction cascade, followed by amadori rearrangement and oxidative modification. Open in New Tab Full Size Figure Download Figure Figure 2 PRISMA flow diagram of study selection proce...
Foreachturnofthecitrateacidcycle,thereisnonetproductionof(A)NADH,(B)FADH2,(C)GTP orATP,(D)citrate,(E)C02. Whenthesubstrateconcentrationofanenzyme-catalyzedreactionisincreasedfrom0.05Kmto0.2 Km,thereactionrateincreases2.5-fold.Howevertherateincreasesonly14%asthesubstrate concentrationisraisedfrom5Kmto20K...
(600 rpm). The reaction was quenched at RT for 1 h by adding 50 µl of quenching buffer (75 mM ethanolamine in 200 mM Tris–HCl/150 mM NaCl, pH 8.5) before dilution (1:12) with the assay buffer (50 mM Tris/300 mM NaCl/2 mM MgCl2/2 mM MnCl2/2 mM CaCl2, pH 7.1) to ...
From a teleological point of view their fate is the completion of capacitation, acrosome reaction, and, after all, the fertilization. Thus, it is possible that most of the biochemical pathways are objective-oriented. In fact these cell must not maintain the cellular homeostasis for long time ...
Thus, the net direction of the lactic dehydrogenase reaction is toward lactate formation rather than lactate utilization. It is concluded that retinal cells use glucose, and not glial derived lactate, as their major substrate.doi:10.1016/S0197-0186(03)00294-8Barry S Winkler...