A,DNep4::HA was expressed under the control of the nativenep4enhancer and labeled with a monospecific anti-HA antibody (nep4 > Nep4::HA).B,ESERCA was labeled with a monospecific antibody detecting the endogenous protein (SERCA). Optical slices of adult heart muscle fibers (A–C) o...
FT CHAIN 2-750 Neprilysin. 分子量 (Da) 85382.74 理论PI值 5.54 CD10/Neprilysin 蛋白结构 NEPRILYSIN COMPLEXED WITH LBQ657 发表文献 2016-04-29 Released: 2016-06-15 作者 Schiering, N., Wiesmann, C. 种属 Homo sapiens 表达系统 Spodoptera frugiperda ...
giving yields of 2.5–3.5 mg/L of transfection culture. SDS-PAGE analysis displayed a single band at approximately 80 kDa for scFc-scFv8D3 (Fig.2A). A strong band at approximately 160 kDa (slightly bigger than that of mouse IgG2c antibody of 150 kDa) was detected for both sNEP-scFc-s...
Cells were then incu- bated in permeabilization buffer (0.1% Triton X-100 in 0.1% sodium citrate) for 2 min on ice. An additional round of washing and centrifugation was performed before incu- bating the cells with anti-NEP antibody (Merck-Millipore; 1:200) in PBS for 25 min at 4 °...
S1 sections were stained either with a mouse monoclonal anti-parvalbumin (1:2500) or a goat anti-somatostatin (1:100), and a rabbit polyclonal anti-Mybpc1 (1:100; Sigma Life Science, St Louis, MO, USA) together with lectin WFA, then counterstained with fluorescent secondary antibody conju...
[2] Voors AA, Dorhout B, van der Meer P. The potential role of valsartan + AHU377 ( LCZ696 ) in the treatment of heart failure. Expert Opin Investig Drugs. 2013 Aug;22(8):1041-7. [3] Laxminarayan G Hegde, Cecile Yu, Cheruvu Madhavi et al. Comparative efficacy of AHU-377, a...