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A,DNep4::HA was expressed under the control of the nativenep4enhancer and labeled with a monospecific anti-HA antibody (nep4 > Nep4::HA).B,ESERCA was labeled with a monospecific antibody detecting the endogenous protein (SERCA). Optical slices of adult heart muscle fibers (A–C) o...
Ninety-six-well half-area plates (Corning Inc., Corning, NY) were coated overnight at 4 °C with 1 μg/mL of one of the following antibodies: 3D6 antibody binding to the N-terminal end of Aβ (recombinantly produced in the lab), m266 antibody binding to the mid-region of Aβ (...
Cells were then incu- bated in permeabilization buffer (0.1% Triton X-100 in 0.1% sodium citrate) for 2 min on ice. An additional round of washing and centrifugation was performed before incu- bating the cells with anti-NEP antibody (Merck-Millipore; 1:200) in PBS for 25 min at 4 °...
The blot was probed with anti-rabbit polyclonal antibody against either unmodified amino-terminus of Ab, N1D (1 mg/ml) or modified amino-terminus of Ab, N3pE (1 mg/ml)16, followed by HRP-conjugated anti-rabbit IgG (GE Healthcare, Tokyo). Immunoreactive bands on the membrane were ...
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Phos Binding Reagent Acrylamide Separation of phosphorylated and non-phosphorylated proteins without phospho-specific antibody Cell Counting Kit-8 (CCK-8) A convenient and sensitive way for cell proliferation assay and cytotoxicity assay Inhibitor Cocktails ...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...
S1 sections were stained either with a mouse monoclonal anti-parvalbumin (1:2500) or a goat anti-somatostatin (1:100), and a rabbit polyclonal anti-Mybpc1 (1:100; Sigma Life Science, St Louis, MO, USA) together with lectin WFA, then counterstained with fluorescent secondary antibody conju...
(R&D Systems) | seven hills bioreagents | zytovision | zyagen Laboratories | ZeptoMetrix | WSU Monoclonal Antibody Center | VMRD | VitaNavi | Virusys | Usbiological | UBPBIO | tulip biolabs | TriLink BioTechnologies | TRC | Ted Pella | swant | steraloids | ST john's laboratory | Sphero...