ESERCA was labeled with a monospecific antibody detecting the endogenous protein (SERCA). Optical slices of adult heart muscle fibers (A–C) or ventral longitudinal muscle fibers (D–F) are shown. Scale bars: 50 µm; ventral
Close to 100 bioreactors with various volume between 2-1000 L to guarantee high-throughput and large-scale production. 重组蛋白表达服务优势 High-efficiency expression vectorsHigh cell density culturingProprietary medium formulation >6000 proteins expression and purification experienceHigh-efficiency expression ...
Triton X-100-extractable membrane fractions (20 mg protein) from the hippocampal formation of APP tg mice with rAAV9-NEPMT or rAAV9-NEPWT and non-transgenic (wild type) mice were subjected to western blot analyses using N-terminal specific antibody N1D (rabbit polyclonal). A single ...
Cells were then incu- bated in permeabilization buffer (0.1% Triton X-100 in 0.1% sodium citrate) for 2 min on ice. An additional round of washing and centrifugation was performed before incu- bating the cells with anti-NEP antibody (Merck-Millipore; 1:200) in PBS for 25 min at 4 °...
(slightly bigger than that of mouse IgG2c antibody of 150 kDa) was detected for both sNEP-scFc-scFv8D3 and muNEP-scFc-scFv8D3. However, bigger bands were also seen for these two proteins, indicating the possible formation of protein multimers (Fig.2A). Structural stability of the ...
S1 sections were stained either with a mouse monoclonal anti-parvalbumin (1:2500) or a goat anti-somatostatin (1:100), and a rabbit polyclonal anti-Mybpc1 (1:100; Sigma Life Science, St Louis, MO, USA) together with lectin WFA, then counterstained with fluorescent secondary antibody conju...