LC-FTMS ESI negative base peak chromatogram for 224C-F2.Cassandra L. QuaveJames T. LylesJeffery S. KavanaughKate NelsonCorey P. ParletHeidi A. CrosbyKristopher P. HeilmannAlexander R. Horswill
(C) Close-up of the residual CPK P2 peaks in the HPLC chromatograms Full size image Table 1 The production of coelimycin P2 by S. coelicolor A3(2) strains after 27 h of culture on solid Glu-MM medium measured as the area under the 9-min peak. The data are shown as the means ...
Fig. 1. The fragmentation behavior of tryptic HeLa and Halo peptide anions as determined via UVPD from the optimized search algorithm in MassMatrix: ion intensities of various product ions normalized by total ion intensities of the spectrum (after precursor and intact charge-reduced peak filtering)...
Precursors were isolated using a ±0.9 Th isolation window, and an exclusion window of ±10 ppm was constructed around the monoisotopic peak of each selected precursor for 45 s. Resolving powers of 60,000 and 15,000 at 400 m/z were used for survey scans and MS/MS scans in the Orbitrap...
Fig. 4: Identification of isotope-labeled metabolites using UHPLC-HR-MS. a Extracted ion chromatograms (EIC, Left) and the isotopic distributions (Right) of glutamine from E. diaphana incubated with 13C6-glucose and 15N-ammonium (Top) and the corresponding glutamine standard (Bottom). The ins...
The RP-HPLC chromatogram of purified peptide showed one major peak at approximate retention time 8.1 min (Fig. 1A). Most of the contaminants and unwanted side products were eluted before 4 min. When the amount of organic solvent was very less (< 10%), the specific fraction ...
corresponds to homodimeric bZIP10 and bZIP25, respectively. Absence of molecular mass peak in and around 24260 Da means that bZIP53 and bZIP10 do not heterodimerize. Figure2Eshow the ESI-MS spectra of mixture of 2 µM each of bZIP53 and bZIP25. Molecular mass peaks that correspond ...
passed these filters, MS1 intensity of the corresponding precursor-ion was extracted using the Philosopher label-free quantification module based on the moFF method (Argentini et al., 2016) (using 10 p.p.m mass tolerance and 0.4 min retention time window for extracted ion chromatogram peak ...
Chemical shifts (δ) for 1H NMR spectra are reported in parts per million (ppm) relative to tetramethylsilane (δ 0.00) or the appropriate residual solvent peak, i.e., CHCl3 (δ 7.27), as internal reference. Multiplicities are given as singlet (s), doublet (d), triplet (t), quartet ...
made their derivatives too polar to elute from the column. This proved useful in subsequent analyses because it allowed the loss of phosphoryl substituents from HepI and HepII to be monitored as the appearance of either a 3-substituted or 3,7-disubstituted heptose derivative peak in the GLC ch...