LC-FTMS ESI negative base peak chromatogram for 224C-F2.Cassandra L. QuaveJames T. LylesJeffery S. KavanaughKate NelsonCorey P. ParletHeidi A. CrosbyKristopher P. HeilmannAlexander R. Horswill
a, HPLC chromatogram of darobactin. Inset, high-resolution mass spectra (HRMS) of darobactin showing a peak at m/z 966.41047, which corresponds to the [M + H]+ ion and another at m/z 483.70865, which corresponds to [M + 2H]2+ ion. b, Higher-energy collisional dissociation–MS/MS...
Precursors were isolated using a ±0.9 Th isolation window, and an exclusion window of ±10 ppm was constructed around the monoisotopic peak of each selected precursor for 45 s. Resolving powers of 60,000 and 15,000 at 400 m/z were used for survey scans and MS/MS scans in the Orbitrap...
The amount of PTH-derivative released in a given sequencing cycle (in picomoles) was determined by comparing the peak height for the amino acid derivative in the chromatogram to the height of its standard peak. I0, the initial amount of a peptide in a sequencing sample (in picomoles), was...
corresponds to homodimeric bZIP10 and bZIP25, respectively. Absence of molecular mass peak in and around 24260 Da means that bZIP53 and bZIP10 do not heterodimerize. Figure2Eshow the ESI-MS spectra of mixture of 2 µM each of bZIP53 and bZIP25. Molecular mass peaks that correspond ...
“peak” Or any impurity “peak.” Solutions of BWFI and BNS, and treprostinil diluted in BWFI and BNS were analyzed using HPLC. FIGS. 1 and 2 shows that the paraben peaks from either methyl- or ethyl-paraben did not interfere (e.g., overlap) with the peak for treprostinil. There ...
Fig. 1. The fragmentation behavior of tryptic HeLa and Halo peptide anions as determined via UVPD from the optimized search algorithm in MassMatrix: ion intensities of various product ions normalized by total ion intensities of the spectrum (after precursor and intact charge-reduced peak filtering)...
Fig. 4: Identification of isotope-labeled metabolites using UHPLC-HR-MS. aExtracted ion chromatograms (EIC, Left) and the isotopic distributions (Right) of glutamine fromE. diaphanaincubated with13C6-glucose and15N-ammonium (Top) and the corresponding glutamine standard (Bottom). The inset corres...
For intracellular Olaparib quantification, analytical HPLC experiments were performed. Olaparib was detected at 218 nm and quantified by measuring the area under the curve (AUC) of the corresponding chromatographic peak. Results are expressed in percentage of the highest AUC value. Figure 5. ...
. Shorter fluorescent lifetimes were also measured in water, which confirmed this character. These dyes were conjugated to a modified polymyxin scaffold that allowed fluorescence “switch-on” upon binding to Gram-negative bacterial membranes, and selective fluorescence detection of bacteria in a wash-...