Double Peaks in Sugar Separations 34. Method Control 35. Mobile Phase pH 36. Signal-to-Noise Improvements 37. Overload 38. Column Durability 39. Fast Analysis and Column Backpressure 40. Column Backflushing 41. Selectivity Shift 42. New Method 43. Negative Peaks 44. Tricky, Tedious, Time ...
HPLC Troubleshooting Symptom Type Baseline disturbance at void time Detector leaks Drifting baseline Ghost peaks High column backpressure Possible Cause Positive/negative—Difference in refractive index of injection solvent Plugged inlet frit Positive direction—Contaminant buildup/elution Positive/negative—...
HPLC Troubleshooting Symptom Type Baseline disturbance at void time Detector leaks Drifting baseline Ghost peaks Possible Cause Positive/negative – Difference in refractive index of injection solvent Plugged inlet frit Positive direction – Contaminant buildup/elution Positive/negative – Difference in ...