OneTaq 一步法 RT-PCR 试剂盒为终点检测 RNA 提供了高效、灵敏的检测方法。使用基因特异性的引物将 cDNA 合成和 PCR 反应放在单管中完成,大大简化 RT-PCR 操作步骤。 该试剂盒包含三种优化的混合液:OneTaq 一步法酶混合液、OneTaq 一步法反应混合液和 OneTaq 一步法 Quick-Load 反应混合液。酶混合液包含 ...
Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
使用LunaScript 一步法多重 RT-PCR 试剂盒(NEB #E1555)或 SuperScript IV One-Step RT-PCR System (Thermo Fisher Scientific) 对人脾总 RNA 的 7 个靶标(185 - 776 bp)进行扩增。取 40% 的 RT-PCR 反应产物进行琼脂糖凝胶(2%)电泳检测。LunaScript 使用标准反应条件:引物终浓度为 1 μM,退火时间为...
Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
Golden Gate Assembly is a versatile, one-step cloning method frequently used for the modular and hierarchical assembly of DNA into large constructs. This article aims to demystify Golden Gate, explains when and how to use it, and gives you everything you need to add this valuable method to ...
探针法定量 PCR(qPCR)原理是利用聚合酶的 5´→3´ 核酸外切酶活性,将淬灭的靶基因特异性探针切断,使其发出荧光,并实时监测释放出的荧光,以测量每个 PCR 循环中的 DNA 扩增。通过检测荧光信号显著超过本底信号时的循环数,可确定 Cq值。Cq值可用于评估评估两个或两个以上的样本之间的相对丰度,也通过梯度稀释已...
NEB 和其它供应商的结果如下所示:Quanta,qScript™XLT 1-Step RT-qPCR ToughMix®;ABI,TaqMan®RNA-to-Ct 1-Step Kit;QIAGEN,QuantiFast®Probe RT-PCR Kit;Bio-Rad,iTaq™Universal Probes One-Step Kit;Promega®,GoTaq®Probe 1-Step RT-qPCR System。NEB 的 Luna 通用探针一步法 RT-qPCR ...
What can I do if a particular amplicon failed in a multiplex reaction using the LunaScript Multiplex One-Step RT-PCR Kit? Can I use the LunaScript Multiplex One-Step RT-PCR Kit to amplify long amplicons? Can the LunaScript Multiplex One-Step RT-PCR Kit be used in the ARTIC workflow for...
Many Initiatives Turning to RT-LAMP as Alternative to PCR for Rapid COVID-19 Screening Assays Citations and protocols featuring LAMP kits Luna®RT-qPCR Kits Luna Universal One-Step RT-qPCR kits offer exceptional sensitivity, reproducibility and RT-qPCR performance. Available for probe- or dye-ba...