In conclusion, we provide in this re- port a highly efficient (90%-100%) and convenient protocol for the deletion of large fragments from either small or large plasmids by one-step cloning. To our knowledge, this is the first report of a site-specific deletion method that can be ...
Described here is an improved protocol of the reported method for one-step PCR site-directed mutagenesis with partial-overlapping primers [1]. The improvements include the new design of partial-overlapping primers, and optimized PCR amplification at the annealing temperature of 60°C, instead of ...
所有PCR相关实验，要调整protocol的时候，一定要做没有调整过的空白作为对照……不然就会像现在这样，解决...
Brochure: Discover Superior RT-PCR results more easily than ever Application note: One-step RT-PCR protocol for amplicon-based viral genome sequencing Featured videos Learn how the SuperScript IV UniPrime One-Step RT-PCR System makes it easy to set up reactions while bri...
Brochure: Discover Superior RT-PCR results more easily than ever Application note: One-step RT-PCR protocol for amplicon-based viral genome sequencing Featured videos Learn how the SuperScript IV UniPrime One-Step RT-PCR System makes it easy to set up reactions while bringing superior...
Application note: One-step RT-PCR protocol for amplicon-based viral genome sequencing Brochure: Discover superior RT-PCR results more easily than ever Product Information User Guide: SuperScript IV UniPrime One-Step RT-PCR System Frequently asked questions (FAQs) ...
Application note: One-step RT-PCR protocol for amplicon-based viral genome sequencing Brochure: Discover superior RT-PCR results more easily than ever Product Information User Guide: SuperScript IV UniPrime One-Step RT-PCR System 常見問答集 (常見問題) The two-phase hot-start mechanism, utilized ...
Alternatively, RT-PCR can be done in two steps, first with the reverse transcription and then the PCR. The two-step protocol is usually more sensitive than the one-step method; yields of rare targets may be improved by using the two-step procedure. ...
The Power SYBR Green RNA-to-CT 1-Step Kit provides high sensitivity and specificity in an easy-to-use one-step qRT-PCR reaction kit. A novel formulation helps reduce false-positive results caused by primer-dimers that ar
Not sure whether to use a one-step or a two-step reverse transcription protocol? We’re here to help. Real-time PCR (qPCR) is a powerful and common technique for accurate analysis of gene expression. When starting with RNA samples, you first need to perform a reverse transcription (RT)...