NEBuilder HiFi DNA Assembly Master Mix has been reformulated with components containing Recombinant Albumin (rAlbumin) beginning with Lot #10238675. 对于大于 15 kb 组装片段的转化,推荐使用 NEB 10-beta E. coli 感受态细胞(高效级,NEB #C3019)。与单独购买 NEBuilder 高保真 DNA 组装预混液和感受态细胞...
NEBuilder Assembly Tool can be used to design primers for your NEBuilder® HiFi DNA Assembly or Gibson Assembly® reactions, based on the entered fragment sequences and the polymerase being used for amplification. NEBridge® Golden Gate Assembly Tool Use this tool to assist with in silico...
NEBUILDER® Assembly Tool 2.0 Fragments Amplified by PCR NEBUILDER® Assembly Tool 2.0 Restriction Enzyme Digest NEBioCalculator® - Using the Ligation module NEBioCalculator® - Using the ds: mass < — > moles module to plan an NEBuilder® HiFi DNA Assembly Reaction Double Digestion with...
NEBuilder HiFi DNA Assembly, Gibson Assembly®, and NEBridge Golden Gate Assembly can be used to create many functional DNA structures, from a simple joining of two metabolic genes, all the way up to the creation of an artificial genome. To help select the best DNA assembly method for ...
What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix? How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase...
兼容NEBuilder HiFi DNA 组装、Gibson 组装以及连接反应NEB Stable 可用于含重复 DNA 片段的质粒提取: 质粒pUC-5xREP 包含 5 个 32 bp 重复序列,因此在重组菌株中不稳定。A) NEB Stable 感受态细胞或 B) Stbl3 感受态细胞使用 2 µl 包含 2.2 ng(0.00125 pmol)pUC19 载体和约 1 ng(0.0028 pmol)5xREP 插...
英文名称 HiFi DNA Assembly Master Mix 储存 -20°C Concentration 2 X 产地 美国 质检 通过质检 售卖区域 全国 服务 提供售前售后服务 发货地 北京 品牌 NEB 价格说明 价格:商品在爱采购的展示标价,具体的成交价格可能因商品参加活动等情况发生变化,也可能随着购买数量不同或所选规格不同而发生变化,如...
NEBuilder Assembly Tool can be used to design primers for NEBuilder HiFi DNA Assembly or Gibson Assembly reactions.
What type of competent cells are suitable for transformation of DNA constructs created using NEBuilder HiFi DNA Assembly Master Mix? How should I store SOC Outgrowth Medium? The SOC I received with my competent cells recommends storage at either room temperature or 4°C, however, when I purchase...
How should fragments be prepared for assembly using NEBuilder HiFi? Are NEB Turbo CompetentE. coli(High Efficiency) or NEB Turbo ElectrocompetentE. colirecommended for cloning repetitive or unstable DNA?