Have you tried NEBuilder HiFi DNA Assembly? NEBuilder HiFi offers several advantages over NEB Gibson Assembly. For more information, visitNEBuilderHiFi.com. Gibson Assembly Workflow Gibson Assembly employs three enzymatic activities in a single-tube reaction: 5´ exonuclease, the 3´ extension activi...
Optimization Tips for NEBuilder® HiFi DNA Assembly and NEB® Gibson Assembly 应用实例 Construction of an sgRNA-Cas9 expression vector via single-stranded DNA oligo bridging of double-stranded DNA fragments Improved method for assembly of linear yeast expression cassettes using NEBuilder® HiFi DNA...
NEBuilder HiFi DNA Assembly, Gibson Assembly®, and NEBridge Golden Gate Assembly can be used to create many functional DNA structures, from a simple joining of two metabolic genes, all the way up to the creation of an artificial genome. To help select the best DNA assembly method for ...
Learn how DNA Assembly, mutagenesis, protein synthesis and purification are compatible with high-throughput workflows and automation.
This tool is ideal for NEB Q5® and NEBuilder® HiFi DNA Assembly applications. Application Note Learn about an accessible method for shuffling DNA using NEBridge® Golden Gate Assembly to recombine proteins and find new properties. Application Note Read about how NEB scientists extract and...
NEBuilder® HiFi DNA Assembly (NEBuilder® 高保真 DNA 组装) NEBuilder®高保真DNA组装基于Gibson无缝组装原理,并在其基础上进行了优化,可实现精准的DNA片段连接。试剂盒通过使用新型高保真聚合酶来实现简单快速的无缝克隆,是合成生物学的绝佳利器。 PART 01 ...
assembly of multiple DNA sequences to create large, artificial DNA structures. To this end, methods have evolved to simplify this process. NEBuilder HiFi DNA Assembly, Gibson Assembly®, and NEBridge Golden Gate Assembly can be used to create many functional DNA structures, from a simple ...
兼容NEBuilder HiFi DNA 组装、Gibson 组装以及连接反应NEB Stable 可用于含重复 DNA 片段的质粒提取: 质粒pUC-5xREP 包含 5 个 32 bp 重复序列,因此在重组菌株中不稳定。A) NEB Stable 感受态细胞或 B) Stbl3 感受态细胞使用 2 µl 包含 2.2 ng(0.00125 pmol)pUC19 载体和约 1 ng(0.0028 pmol)5xREP 插...
What type of competent cells are suitable for transformation of DNA constructs created using Gibson Assembly? Which strain of Competent E.coli should I use for general cloning? Which kind of transformation tubes should be used? What volume of DNA can be added into competent cells? Are NEB's ...
Gibson Assembly®, Cloning Ligation 产品组分信息 本产品提供以下试剂或组分: NEB #名称组分货号储存温度数量浓度 M0208V -20 Taq DNA Ligase M0208VVIAL -20 1 x 0.025 ml 40,000 units/ml Taq DNA Ligase Reaction Buffer B0208SVIAL -20 1 x 1.5 ml 10 X M0208S -20 Taq DNA Ligase...