NEBuilder HiFi offers several advantages over NEB Gibson Assembly. For more information, visit NEBuilderHiFi.com. Gibson Assembly Workflow Gibson Assembly employs three enzymatic activities in a single-tube reaction: 5´ exonuclease, the 3´ extension activity of a DNA polymerase and DNA ligase ...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. ...
Introduction to NEBUILDER HIFI DNA ASSEMBLY® Find out how NEBuilder® HiFi DNA Assembly can reliably join DNA fragments in a single tube, isothermal reaction, with advantages over NEB Gibson Assembly®. Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly®or Gibson Assembly...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. Watc...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. ...
NEBuilder® HiFi DNA Assembly (NEBuilder® 高保真 DNA 组装) NEBuilder®高保真DNA组装基于Gibson无缝组装原理,并在其基础上进行了优化,可实现精准的DNA片段连接。试剂盒通过使用新型高保真聚合酶来实现简单快速的无缝克隆,是合成生物学的绝佳利器。 PART 01 ...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. ...
NEBuilder HiFi DNA Assembly, Gibson Assembly®, and NEBridge Golden Gate Assembly can be used to create many functional DNA structures, from a simple joining of two metabolic genes, all the way up to the creation of an artificial genome. To help select the best DNA assembly method for ...
NEBuilder® 组装工具 可以使用 NEBuilder 组装工具d 根据输入的片段序列和用于扩增的聚合酶,为您的 NEBuilder® HiFi DNA Assembly 或 Gibson Assembly® 反应设计引物。 NEBridge™ 金门组装工具使用此工具协助进行 Golden Gate DNA 组装的计算机 DNA 构建设计。它能够准确设计具有适当类型 IIS 限制位点和重叠的...
兼容NEBuilder HiFi DNA 组装、Gibson 组装以及连接反应 NEB Stable 可用于含重复 DNA 片段的质粒提取: 质粒pUC-5xREP 包含 5 个 32 bp 重复序列,因此在重组菌株中不稳定。A) NEB Stable 感受态细胞或 B) Stbl3 感受态细胞使用 2 µl 包含 2.2 ng(0.00125 pmol)pUC19 载体和约 1 ng(0.0028 pmol)5xREP...