英文名称 HiFi DNA Assembly Master Mix 储存 -20°C Concentration 2 X 产地 美国 质检 通过质检 售卖区域 全国 服务 提供售前售后服务 发货地 北京 品牌 NEB 价格说明 价格:商品在爱采购的展示标价,具体的成交价格可能因商品参加活动等情况发生变化,也可能随着购买数量不同或所选规格不同而发生变化,如...
NEBuilder HiFi DNA Assembly Master Mix(NEB #E2621) Does not include competent cells Online Supporting Tools NEBuilder®Assembly Tool Use to easily design primers for your NEBuilder HiFi DNA Assembly reactions. NEBuilder®Protocol Calculator
During assembly, the T5 exonuclease activity chews back the 5-prime end of the DNA fragment, generating a 3-prime single-stranded overhang, which allows the oligo to anneal with it. The high-fidelity DNA polymerase activity fills the gap, and DNA ligase seals the nicks. The T5 ...
coli (NEB #C3040) should be used.NEBuilder has been used in various applications, including: Site-directed mutagenesis Construction of an sgRNA-Cas9 expression vector | Animation Assembly of linear yeast expression cassettes To help select the best DNA assembly method for your needs, please use...
65°C, and a lower primer concentration of 150 nM. Reaction Assembly Cat No.95200-025 95200-100 95200-500 Size: 25 x 25 µL reactions (1 x 0.625 mL)100 x 25 µL reactions (1 x 2.50 mL) 500 x 25 µL reactions (1 x 12.50 mL) Store at -25°C to - 15°C ...
coli (NEB #C3040) should be used.NEBuilder has been used in various applications including: Site-directed mutagenesis Construction of an sgRNA-Cas9 expression vector | Animation Assembly of linear yeast expression cassettes To help select the best DNA assembly method for your needs, please use ...