NEBuilder HiFi DNA Assembly Master Mix(NEB #E2621) Does not include competent cells Online Supporting Tools NEBuilder®Assembly Tool Use to easily design primers for your NEBuilder HiFi DNA Assembly reactions. NEBuilder®Protocol Calculator
产品名称 高保真 DNA 组装预混液 产品规格 50 reactions 型号 E2621L 用途范围 用于科研 是否进口 是 加工定制 否 英文名称 HiFi DNA Assembly Master Mix 储存 -20°C Concentration 2 X 产地 美国 质检 通过质检 售卖区域 全国 服务 提供售前售后服务 发货地 北京 品牌 NEB 价格说明 价格:商...
Introduction to Gibson Assembly® Watch an interactive tutorial that details the process by which Gibson Assembly® joins DNA fragments in a single tube, isothermal reaction. Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly® or Gibson Assembly® Watch an interactive tutorial...
coli 感受态细胞转化效率的影响:按照所提供的高效级转化操作流程,用 100 pg pUC19 对照 DNA 转化 50 μl 感受态细胞,DNA 温育时间的变化范围为 0 至 40 分钟。细胞复苏培养基对转化效率的影响:按照所提供的高效级转化操作流程,用 100 pg pUC19 对照 DNA 转化 50 μl NEB 5-alpha E.coli 感受态细胞,不同...
Closer to the clinic: Optimized DNA assembly for personalized medicine research In this webinar, we will discuss how data-optimized Golden Gate Assembly and NEBuilder® HiFi DNA Assembly mixes and protocols can be used to enable new and massively parallel approaches to personalized medicine. ...
Primer Design and Fragment Assembly Using NEBuilder HiFi DNA Assembly® or Gibson Assembly® Watch an interactive tutorial on primer design to see how simple it really is to clone with either NEBuilder HiFi DNA Assembly or the Gibson Assembly Cloning Kit. TIME-SAVER™ Qualified Restriction Enzy...
NEBuilder HiFi DNA Assembly Master Mix was developed to improve the efficiency and accuracy of DNA assembly. This method allows for seamless assembly of multiple DNA fragments, regardless of fragment length or end compatibility. This method has been used to assemble either single-stranded oligonucleotid...
兼容NEBuilder HiFi DNA 组装、Gibson 组装以及连接反应 NEB Stable 可用于含重复 DNA 片段的质粒提取: 质粒pUC-5xREP 包含 5 个 32 bp 重复序列,因此在重组菌株中不稳定。A) NEB Stable 感受态细胞或 B) Stbl3 感受态细胞使用 2 µl 包含 2.2 ng(0.00125 pmol)pUC19 载体和约 1 ng(0.0028 pmol)5xREP...
Supplemental Protocol 1: Generation of DNA fragments by PCR assembly of pooled oligos (NEB #M0689) Supplemental Protocol 2: Using colony PCR to identify positive clones (NEB #M0689) 说明书 产品说明书包含产品使用的详细信息、产品配方和质控分析。 manualM0689工具...
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