Small ncRNA-Seq Results of Human Tissues: Variations Depending on Sample IntegrityBACKGROUND: Although mature miRNAs are relatively stable in vivo, RNA degradation can have a substantial influence on small noncoding RNA (sncRNA) profiles.Ludwig, Nicole...
识别新的ncRNA利用CRIST-seq的高灵敏度,我们能发现并识别新的ncRNA,这些RNA在常规的全细胞RNA表达筛选中可能被忽视。此方法对于揭示多种ncRNA如何具体调控特定基因的表达提供了新视角。 研究各类细胞中的基因调控CRIST-seq能用于研究正常细胞、干细胞以及癌细胞中基因表达的ncRNA调控机制,为我们理解疾病的发生机制提供了新...
CRISTseq是一种创新的分子生物学和基因组学方法，用于鉴定和分析与特定基因调控相关的非编码RNA相互作用网络。技术原理：CRISTseq结合了核内原位逆转录与ncRNA分子的生物素标记，并利用催化失活的CRISPR dCas9基因靶向的特异性，实现对目标基因位点的ncRNA相互作用网络的全面研究。应用价值：该方法为基因表达调...
——DRIPc-seq对形成R-loop的RNA进行链特异性建库和测序分析,有效确定形成R-loop的lncRNA或mRNA,更精准的检测全基因组水平的R-loop分布 联合lncRNA芯片,助力lncRNA深入研究 即将推出针对 circRNA的DRIPc-seq, 敬请关注公司网站及公众号! 讲座安排: 2023.03.30(周四) 14:30--16:00...
Three files must be ready to run PathogenTrack: 1) the valid barcode.tsv file; 2) the raw scRNA-seq fastq file (xxx_R1.fastq.gz; xxx_R2.fastq.gz).Then we run PathogenTrack to identify and quantify pathogen expression at the single-cell level:...
starBase v2.0: decoding miRNA-ceRNA,miRNA-ncRNA and protein–RNA interactionnetworks from large-scale CLIP-Seq dataJun-Hao Li, Shun Liu, Hui Zhou, Liang-Hu Qu* and Jian-Hua Yang*RNA Information Center, Key Laboratory of Gene Engineering of the Ministry of Education, State KeyLaboratory for ...
内容提示: starBase v2.0: decoding miRNA-ceRNA,miRNA-ncRNA and protein–RNA interactionnetworks from large-scale CLIP-Seq dataJun-Hao Li, Shun Liu, Hui Zhou, Liang-Hu Qu* and Jian-Hua Yang*RNA Information Center, Key Laboratory of Gene Engineering of the Ministry of Education, State Key...
dividual miRNA-ncRNA and protein–RNA interactions, generalizing these findings to thousands of RNAs remains a daunting challenge. Recent advances in high-throughput sequencing of immunoprecipitated RNAs after cross-linking (CLIP- Seq, HITS-CLIP, PAR-CLIP, CLASH, iCLIP) provide powerful ways to ...
S. Martens-Uzunova, "FlaiMap- per: computational annotation of small ncRNA-derived frag- ments using RNA-seq high-throughput data," Bioinformatics, vol. 31, no. 5, pp. 665-673, 2015.Hoogstrate,Y. et al. (2015) FlaiMapper: computational annotation of small ncRNA-derived fragments using ...
et al. (2014) FlaiMapper: computational annotation of small ncRNA-derived fragments using RNA-seq high-throughput data, Bioinformatics., 31, 665-673Hoogstrate Y, Jenster G and Martens-Uzunova ES. FlaiMapper: computational annotation of small ncRNA derived fragments using RNA-seq high throughput ...