Natural productbioactive compoundPKSNRPSgene clusterbiosynthetic pathwaysecondary metabolismNatural products synthesized by polyketide synthase (PKS), non-ribosomal peptide synthetase (NRPS) and the hybrid PKS-NRPS have been valuable sources for bioactive compounds due to their structural diversity and wide ...
biosynthetic pathway (hit rate of building blocks). We introduced the hit rate of building blocks because the pathway of ground truth is not necessarily unique. There may be multiple pathways between a natural product and its building blocks. Even if a candidate pathway is not the ground truth...
An integrative approach has now enabled elucidation of the complete biosynthetic pathway of a prominent saponin adjuvant. Reconstruction of the whole biosynthetic pathway in a heterologous host provides new perspectives for the biotechnological supply of this immunostimulant. ...
The discovery was made by a collaborative group of chemists at the University of Illinois and Harvard University. Together, they confirmed that two otherwise unrelated, bacterially-produced compounds shared an unusual set of steps in their biosynthetic pathways. Deciphering this type of biochemical proce...
Identification, cloning, expression and functional interrogation of the biosynthetic pathway of the polychlorinated triphenyls ambigol A–C from Fischerella ambigua 108b Org Chem Front, 7 (2020), pp. 3193-3201 View articleCrossrefView in ScopusGoogle Scholar 61 V. Agarwal, A.A. El Gamal, K....
Fig. 12. Biosynthetic pathway of 3-thiaglutamate (24). (A) 3-thiaglutamate (24) formation catalyzed by Tgl enzymes. (B) Cysteine addition by the PEARL TglB to the leader peptide TglA. The reaction starts with phosphorylation of the C-terminal carboxylate of TglA, which then forms an amid...
et al.2011), suggesting that even higher yields are feasible. Crucial factors for optimization of recombinant rhamnolipid production inP. putidainclude the choice of an appropriate promoter and the increase in biosynthetic flux toward the product, e.g., by deletion of the competing PHA pathway. ...
thus greatly contributing to pathway divergence in natural product biosynthesis. As the functions and diversity of core synth(et)ases are well summarized elsewhere [1,2,4], this review focuses on the biosynthetic processes in which pathway branching occurs at the mid- or late-biosynthesis stage, ...
Discovery of McbB, an enzyme catalyzing the β-carboline skeleton construction in the marinacarboline biosynthetic pathway. Angew. Chem. Int. Ed Engl. 52, 9980–9984 (2013). CAS PubMed Google Scholar Nikolouli, K. & Mossialos, D. Bioactive compounds synthesized by non-ribosomal peptide ...
Here we focused on a conserved fungal biosynthetic pathway that lacks a canonical core enzyme and used heterologous expression to identify the associated natural product, a highly modified cyclo-arginine-tyrosine dipeptide. Biochemical characterization of the pathway led to identification of a new ...