The engineering production of Nbs has evolved into a mature process, involving library construction, screening, and expression purification. Different library types, including immune, nave, and synthetic/semi-synthetic libraries, offer diverse options for various applications, while display platforms like ...
Next, we examined the potential impact that our nanobody sequences may have on immunogenicity in humans, as a major concern related to the therapeutic use of nanobody is the possibility that, as camelid proteins, they would elicit an immune response. In particular, VHH hallmark residues in fra...
KMD Bioscience has a mature recombinant antibody library construction system to provide customers with high quality VHH naïve and immune library construction ser... Read More Aptamer Synthesis Platform KMD Bioscience has a mature aptamer synthesis platform and has accumulated a lot of experience in ...
The ELISA plate was coated with the PCSK9 antigen, the phage display technology was used to screen the antibody library of PCSK9 immune single domain antibodies, and then the screened single domain antibodies were transformed into E. coli expression systems for mass expression, so that the ...
Rational design of chimeric antigen receptor T (CAR-T) cells based on the recognition of antigenic epitopes capable of evoking the most potent CAR activation is an important objective in optimizing immune therapy. In solid tumors, the B7-H3 transmembrane protein is an emerging target that harbour...
Construction of the VHH library The titres of antibody against PPV-VP2 protein in the serum samples from the immunized camel reached 1:512,000 (Fig. 2a), suggesting that the camel produced a good immune response to the PPV-VP2 protein. After amplification, ligation, and transformation, a ph...
naïve library, peripheral blood lymphocytes are taken from non-immunized donors. The naïve library avoids the immunization step and takes advantage of the high diversity of the host’s immune system. Because there is no affinity maturation step, such as in vivo immunization, a large pool of...
VHH-72BivalentVHH-72-Fc Immune library + phage display, IgG Fc fusion SARS-CoV-1MERS-CoVSARS-CoV-2 RBD 36.8 nM 13.3 nM Recognize epitope residues (Trp100, Tyr356/494, Cys366, Phe 364,Ser358,Arg 426) on spike RBD,Blocks RBD-ACE2 interaction,Neutralized SARS-CoV-2 pseudovirus...
Moreover, MY2935, a Nb97-Nb97-HSA fusion protein (Nb97, anti-PD-1 nanobody) exerted favorable effects on blocking PD-1/PD-L1 pathway and thus stimulating T cell associated antitumor immune response [98]. In our laboratory, Wang et al. constructed the fusion protein Fv-LDP-D3 and its...
The variable central portion of flagellin has been a favourite target site for insertion of heterologous peptides or small proteins to display them on the cell surface for studying binding interactions and immune responses11,12,13. For example, Lu et al. created the FliTrx thioredoxin-flagellin ...