随着重组DNA技术的出现,许多抗体片段已经被开发出来。其中,骆驼重链可变区(VHHs)和单链可变区(ScFv)是最受青睐的可变区。在单链抗体被广泛应用的同时,骆驼纳米抗体(nanobodies)由于具有更高的溶解度、稳定性、更小的尺寸和更低的生产成本等优异的化学和物理特性,...
Nanobodies can provide specific binding to divergent antigens, leading to many promising therapeutic and detection applications in recent years. Traditional technologies of nanobody discovery based on alpaca immunization and phage display are very time-c
as linker candidates, primarily based on thelinkerdbwwwlinker database27. The aGFP_ENH nanobody, with linkers from the chosen set randomly attached to its both ends (Fig.1b-c), was inserted into flagellin replacing the D3 domain. In this way a...
192,193,194,195,196]. These databases not only provide structured and high-quality data but also support critical tasks, such as sequence-function relationship analysis, structure prediction, and thermal stability optimization. By integrating these resources, AI frameworks can bridge the gap between e...
These unique antibody fragments interact with antigen by virtue of only one single variable domain, referred to as VHH or nanobody. Several characteristics make nanobody use superior to the abovementioned antibodies. They are non-immunogenic and show high thermal and chemical stability. There are ...
The article reports on the announcement of biopharmaceutical company Ablynx that its nanobodies have been selected by Boehringer Ingelheim for the further development of Alzheimer's disease treatment.EBSCO_bspPharmawatch Biotechnology
The protein sequences of VHHs were obtained from the sdAb-DB, PDB and NCBI database [17]. The multiple sequence alignment was performed with CLC sequence view 8 software. The sequence logos of amino acid frequency in the CDR1 and CDR2 were evaluated by Web-Logo [18]. The protein solubi...
2.8.1. Database search for the motif RXE A python code was written to analyze all non-redundant protein sequences from NCBI's BLAST database (with entries from GenPept, Swissprot, PIR, PDF, PDB, and RefSeq; downloaded June 14, 2021, at: https://ftp.ncbi.nlm.nih.gov/blast/db/). Br...
Ultrasonic nanobubbles carrying anti-PSMA nanobody:construction and application in prostate cancer-targeted imaging. Fan X,Wang L,Guo Y,et al. PLo S One . 2015Fan, X. et al. (2015) Ultrasonic nanobubbles carrying anti-PSMA nanobody: construction and application in prostate cancer-targeted ...
A rapid and sensitive direct competitive fluorescence enzyme immunoassay (dc-FEIA) for detecting 3-PBA on the basis of a nanobody (Nb)-alkaline phosphatase (AP) fusion protein was developed. The anti-3-PBA Nb-AP fusion protein was expressed and purified. The 50% inhibitory concentration (...