红色荧光蛋白目的:建立猪naive-like诱导性多能干(induced pluripotent stem,i PS)细胞系,并对其进行红色荧光标记,为通过示踪猪naive i PS细胞发育和分化的相关研究奠定基础.方法:首先利用核转染技术向巴马小型猪胎儿成纤维细胞(porcine embryonic fibroblast,PEF)中转入鼠源OCT4,SOX2,KLF4和c-MYC转录因子表达载体Tet O...
分化所得到的神经细胞具有明显的神经细胞结构特征rtpcr和免疫荧光结果显示分化后的神经细胞表达多种神经细胞特异性基因qpcr结果显示分化后多能因子的表达显著降低神经特异性基因表达显著升高免疫荧光结果经统计分析后显示低分子量神经微丝蛋白lightneurofilamentproteinnfl阳性细胞率可达79 猪naive-like 胚胎干细胞向神经细胞...
naive:adj天真的 white：adj. 白色的，纯洁的; 无色的，透明的; 白衣的; 白种人的;n. 白色; 白种人; 空白; 白色颜料;vt. （书写，印刷等） 留出空白处; 使变白色，刷白; 漂白;like:vt. 喜欢; （与 would 或 should 连用表示客气） 想; 想要; 喜欢做;prep. （表示属性）...
目的:建立猪naive-like诱导性多能干(induced pluripotent stem,i PS)细胞系,并对其进行红色荧光标记,为通过示踪猪naive i PS细胞发育和分化的相关研究奠定基础.方法:首先利用核转染技术向巴马小型猪胎儿成纤维细胞(porcine embryonic fibroblast,PEF)中转入鼠源OCT4,SOX2,KLF4和c-MYC转录因子表达载体Tet O-FUW-OS...
(ESC) and EpiSC states can transit between each other 6 . Although pluripotency in the embryo is a transient condition, it can be extended inde�initely in vitro, through derivation o� ESCs �rom the ICM 7,8 . In mice, this state is re�erred as ground- or naive-state ...
Singh, C. Sun et al., "Isolation and cultivation of naive-like human pluripotent stem cells based on HERVH expression," Nature Protocols, vol. 11, no. 2, pp. 327-346, 2016.Wang J, Singh M, Sun C, Besser D, Prigione A, Ivics Z, et al. Isolation and cultivation of naive-like ...
Antigen-primed B cells return to a naive-like state As we detected no significant apoptosis of B cells transiently primed with DEL-OVA in the absence of T-cell helpin vivo, we asked whether these B cells remained activated and capable of receiving T-cell help. At 12 h after their tran...
naive-ui-like-nuxt3-template是一个基于Nuxt3的前端模板库,灵感来源于Naive UI。它提供了一套美观、简洁的组件和布局,可用于快速构建现代化的Web应用程序。该模板库结合了Nuxt3的强大功能和Naive UI的优雅设计,使开发者能够以更高效、更舒适的方式开发应用。 该模板库包含了多个可复用的组件,如按钮、表单、导航...
Naive embryonic stem cells hold great promise for research and therapeutics as they have broad and robust developmental potential. While such cells are readily derived from mouse blastocysts it has not been possible to isolate human equivalents easily, although human naive-like cells have been artifici...