1C, one-cell; E2C, early two-cell; L2C, late two-cell. Source data Full size image GABPA is a member of the erythroblast transformation specific (ETS) TF family, which forms a tetrameric complex with GABPB to regulate the transcriptional activation20. Previous studies have shown that ...
et al. Whole-genome bisulfite sequencing of two distinct interconvertible DNA methylomes of mouse embryonic stem cells. Cell Stem Cell 13, 360–369 (2013). Article CAS PubMed Google Scholar Hackett, J. A. et al. Synergistic mechanisms of DNA demethylation during transition to ground-state ...
Human periimplantation development requires the transformation of the naive pluripotent epiblast into a polarized epithelium. Lumenogenesis plays a critical role in this process, as the epiblast undergoes rosette formation and lumen expansion to form the amniotic cavity. Here, we present a high-...
The PCR products were then ligated to the T vector using the pEASY-T1 Cloning Kit, followed by transformation into DH5α E. coli on plates. After 16 h, 15 to 20 individual clones were selected and sequenced. By calculating the number of different clones, the distinct genotypes of SNPs in...
For example, slow naive T cell recovery in lymphopenic humans is thought to be caused by age related thymic atrophy (Crooks et al., 2006), and long-term failure of immune reconstitution after stem cell transplantation (SCT) has been related to exhaustion of thymic output (Patel et al., ...
Assembly of a stem cell-derived human postimplantation embryo model Article11 September 2024 Introduction Pluripotent stem cells (PSCs) possess the unique ability to self-renew and differentiate into all cell types of a fully functional adult, making them invaluable tools to study human development,...
cell intermediates and enables somatic cell reprogramming in absence of otherwise essential pluripotency medium requisites. Activation of FGF-ERK signalling, which promotes exit of naïve pluripotent cells from self-renewal, does not prevent JAK/STAT3 induced post-implantation epiblast-derived stem cell ...
48 The embedding shows coherent developmental progression for 15 cell types and stages, including the pluripotent lineage from morula to ICM, pre- and post-implantation epiblast (Figure 2A). We used UMAP transformation to map PSC samples onto the embedding space. This algorithm positions query ...
EBNA-LP has auxiliary, supportive functions in B-cell activation and stable latent infection. The function of EBNA2 is well defined, but the contribution of EBNA-LP to B-cell activation and transformation is less clear. Early experiments with a C-terminally truncated EBNA-LP indicated that its...
et al. Tet2 loss leads to increased hematopoietic stem cell self-renewal and myeloid transformation. Cancer Cell 20, 11–24 (2011). CAS PubMed PubMed Central Google Scholar Zhang, Q. et al. Differential regulation of the ten-eleven translocation (TET) family of dioxygenases by O-linked ...