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英文名称:Naive Pan T Cell Isolation Kit, human 其它信息:包装信息:EA | 备注: 见优宁维官网 Miltenyi Biotec Asia Pacific Pte Ltd 联系电话:65-6238-8183 电子邮件:macs@miltenyibiotec.com.sg CB指数:58 网址:www.miltenyibiotec.com 相关信息:产品目录(3230)用户评价 ...
A mouse Pan T Cell Isolation Kit II (Miltenyi Biotec) was used to isolate mouse T cells. A Mouse sTNFR1 ELISA Kit (RayBiotech) was used for analyzing soluble TNFR1 in mouse serum samples. Plasmids Mouse Ampd3 was cloned into a p3xFLAG-CMV14 vector (Sigma-Aldrich). Individual Ampd3 mu...
PBTs or naive CD4+ T cells were isolated from PBMCs using a Pan-T-cell isolation kit or naive CD4+ T-cell isolation kit (Miltenyi Biotec, Bergisch Gladbach). This study was approved by the Ethics Committee of Heidelberg University (S-089/2015). Keratinocyte/T cell coculture KCs, either ...
Isolation of CD3+, CD4+, and CD8+ lymphocytes was performed using Invitrogen Dynabeads© negative isolation kit selection according to the manufacturer’s instructions (Thermofischer, USA), and resulted in a purity of 90–95% for the three cell types. Isolated cell were characterized as mainly...
Cell isolation Lymph node single cell suspensions were prepared from peripheral mouse LNs or thymus. CD4+ and CD8+ T cells were negatively selected using the Dynal bead mouse CD4- or CD8-negative isolation kit (Invitrogen Corp., Carlsbad, CA) according to the manufacturer's protocol. The purity...
CD4 þ and CD8 þ T cells were negatively selected using the Dynal bead mouse CD4- or CD8-negative isolation kit (Invitrogen Corp., Carlsbad, CA) according to the manufacturer's protocol. The purity of each cell population was 498% as determined by flow cytometry. Cell culture and ...
Cell attachment proteins and peptides Geltrex Thermo Fisher Scientific Cat#A1413302 Laminin Millipore Cat#CC095-5MG Fibronectin Millipore Cat#FC010 Laminin 111-E8 From Kiyotoshi Sekiguchi N/A Critical commercial kits Alkaline Phosphatase Kit Sigma-Aldrich Cat#86R-1KT Neon 10ul transfection kit Thermo...
Systemic lupus erythematosus (SLE) is the quintessential autoimmune disease, as it is characterized by hyperactivity of CD4+T cells and subsequently drives lupus pathology. Follicular helper T (TFH) cells play an important role in B cell maturation and antibody production. However, which specific ...
PBMC cell isolation and plasma collection Peripheral blood samples were collected in heparin sodium tubes and processed within 6 h of collection. PBMCs were isolated by density gradient centrifugation at 1,000gfor 10 min. Aliquots from the plasma layer were collected and stored at −80 ...