该试剂盒通过两步法,从人PBMC中分离未经标记的初始CD8T细胞。首先,通过生物素标记抗体和抗生物素微球的混合物标记非初始CD8T细胞和NK细胞,从而得到预富集的初始CD8T细胞。第二步,富集到的初始CD8T细胞被CD8磁珠标记,进一步正选出初始CD8细胞。 优点: -简单、高效; ...
该试剂盒用于从小鼠脾脏和淋巴结单细胞悬液中分离未经经标记的初始CD8+T细胞。 详细的分离过程 为了去除记忆CD8 + T细胞和非CD8 + T细胞,将单细胞悬液与生物素化CD45R,CD4,CD11b,CD11c,CD19,CD44,CD49b,CD105,Ter-119,MHC II和 -TCRγ/δ抗体混合物孵育。 随后将这些细胞用抗生物素微珠进行磁性标记从而被...
Customers Also Purchased MojoSort™ Human CD4 T Cell Isolation Kit MojoSort™ Human CD3 T Cell Isolation Kit MojoSort™ Mouse CD4 Naïve T Cell Isolation Kit MojoSort™ Human CD8 T Cell Isolation Kit Version: 2 Revision Date: 01/26/2022 POPULAR...
销售价:¥ 9103.62/ 件 市场价:¥ 10710.14/ 件 在线询价 优惠:请询价 订货号:1DV5158 品牌型号:美天旎 130-096-543 货期:询货期 最小订货量:1件 +- 美天旎 联系我们 400-920-3909 微信扫一扫,手机采购 产品介绍Product Description The Naive CD8a+T Cell Isolation Kit was developed for the isola...
Easily and efficiently isolate highly purified human naïve pan T cells from fresh or previously frozen human peripheral blood mononuclear cells (PBMCs) or washed leukapheresis samples by immunomagnetic negative selection, with the EasySep™ Human Naïve Pan T Cell Isolation Kit. Widely used in ...
Isolation of naive and memory CD8 T cells Naive CD8 T cells were enriched by MACS positive selection using the naive CD8 T isolation kit (AutoMACS; Miltenyi Biotec), while memory CD8 T cells by MACS negative selection using the memory CD8 T isolation kit (AutoMACS; Miltenyi Biotec) according...
The MojoSort™ Mouse CD4 Naïve T cell Isolation Kit was used to isolate cells from C57BL/6 mouse spleen. Top: CD4+T cells after isolation. Middle: CD4+ Naïve T cells after isolation, defined by the CD62L (clone: MEL-14) PE and CD44 (clone: IM7) FITC profile. Bottom: cells...
Naive CD8+ T cells from OT1 mice were isolated from spleen and lymph nodes using the CD8+ T-cell isolation kit (Miltenyi Biotech), followed by 2 additional purification steps: depletion of DCs by CD11c+ microbeads, and then positive selection of naive CD62Lhigh cells using CD62L+ microbead...
p47phoxÀ/À mice have LN hyperplasia, increased total numbers of B and T lympho- cytes and lower T : B cell ratios than age- and sex-matched wild-type (WT) mice (Figure 1). However, p47phoxÀ/À and WT CD4 : CD8 ratios were comparable. Flow-cytometry analysis showed p47...
Cell isolation Lymph node single cell suspensions were prepared from peripheral mouse LNs or thymus. CD4+ and CD8+ T cells were negatively selected using the Dynal bead mouse CD4- or CD8-negative isolation kit (Invitrogen Corp., Carlsbad, CA) according to the manufacturer's protocol. The purity...