Naïve CD4+ T cells were isolated from mouse splenocytes using the EasySep™ Mouse Naive CD4+ T Cell Isolation Kit (Catalog #19765), activated with plate-bound anti-CD3 and soluble anti-CD28, and cultured in medium alone, or medium containing ImmunoCult™ Mouse Treg Differentiation Suppl...
然后使用EasySep™PE选择混合物选择预先用CD62L PE标记的CD4+ CD62L+细胞。 所需的细胞用抗体和磁珠。 使用EasySep™磁极将细胞分离而不用分选柱。对于通过阴性选择在短至15分钟内分离小鼠初始CD4+ T细胞,我们推荐使用EasySep™小鼠初始CD4+ T细胞分离试剂盒(19765)。 优点:...
First, naive CD4+ T cells are pre-enriched by negative selection using the EasySep™ Mouse Naïve CD4+ T Cell Pre-Enrichment Cocktail containing biotinylated antibodies directed against non-naïve CD4+ T cells (CD8, CD11b, CD11c, CD19, CD24, CD25, CD45R, CD49b, TCRγ/δ, TER119)....
规格:1 Kit Stemcell 18765 EasySep™ Mouse CD4+CD62L+ T Cell Isolation Kit Product Overview Isolate highly purified naïve CD4+ T cells from single-cell suspensions of splenocytes or other tissues by using a simple, two-step method. First, naive CD4+ T cells are pre-enriched by negati...
然后使用EasySep™PE选择混合物选择预先用CD62L PE标记的CD4+ CD62L+细胞。 所需的细胞用抗体和磁珠。 使用EasySep™磁极将细胞分离而不用分选柱。对于通过阴性选择在短至15分钟内分离小鼠初始CD4+ T细胞,我们推荐使用EasySep™小鼠初始CD4+ T细胞分离试剂盒(19765)。 优点:...