This notion is readily observed with TCR transgenic cells of high affinity such as OT-I; these cells are generated in large numbers and, on transfer, undergo a faster rate of LIP than cells of lower-affinity TCR, such as 2C or P14 (Kieper et al., 2004). Conversely, some very low-...
(F) Representative colonies stained for AP activity of Prdm14+/+, Prdm14−/−, Prdm14−/−: nGFP, and Prdm14−/−: AG-P14 cells in 2i. Bar, 20 μm. (G) AP-positive colony formation by Prdm14+/+, Prdm14−/−, Prdm14−/−: nGFP, and Prdm14−/−: AG-...