Here we provide evidence that human tonsillar GC B cells, as compared to nave tonsillar B cells, may have a higher threshold for affinity selection but be better able to interact with T cells and, once triggered through the BCR, may form more stable signaling complexes. A comprehensive ...
The perspective that naive B-cell recognition of antigen in the absence of T-cell help causes cell death or anergy is supported by in vivo studies of B cells that are continuously exposed to self-antigens. However, intravital imaging suggests that early
Ighb strains had larger numbers of PE-specific naive B cells and generated smaller germinal center responses and larger numbers of IgM memory cells than the Igha strain. The properties of PE-specific B cells in Ighb mice correlated with usage of a single VH that afforded high-affinity PE ...
什么是Naïve B cell 与T细胞类似,B细胞也是从干细胞分化而来,通过接触独特的抗原分化后激活。B细胞表面受体BCR与外来抗原结合后发挥下游生物学功能。通常来说,B细胞发育的最后阶段在脾脏中,在那里发育成初始B细胞。这些不被激活的初始B细胞随着血液进入到刺激淋巴器官(SLO)。初始的B细胞更容易捕获病原体,因为SLO的...
(naive B cells, for example) could be targeted by antibodies with specificity to nucleocapsid or RBD, or could have no discernable affinity to the dominant viral antigens tested (Fig.3a). Instead, the data presented here are most consistent with a model by which the highly inflammatory milieu...
P Reichardt,B Dornbach,S Rong,S Beissert,M Gunzer 摘要: Naive B cells are ineffective antigen-presenting cells and are considered unable to activate naive T cells. However, antigen-specific contact of these cells leads to stable cell pairs that remain associated over hours in vivo. The ...
The IFN-λ1-induced pSTAT1 level is slightly higher in naive B cells compared with CD27+ memory B cells (Figure S1F). A JAK inhibitor assay was used to confirm that IFN-λ signals through the JAK-STAT pathway: the JAK inhibitor (3 μM ruxolitinib) almost blocked IFN-α2- or IFN-...
摘要: Objectives To examine the expression of B-cell-activating factor receptor (BAFF-R) on naive CD27− and memory CD27+ B cells in normal individuals and patients with rheumatoid arthritis (RA) undergoing B-cell depletion therapy with rituximab....
Resting (CD38low) tonsillar B cells differentiate to express the centroblast-restricted CD77/globotriaosylceramide antigen on high-level engagement of CD154. As the CD38low population comprises both naive and memory subsets, we wished to compare the propensity of each to develop this germinal ...
aThe ABCB1 Transporter is also used to differentiate Transitional B-cells from Naive B-cells. Dyes such as Rhodamine123 and MitoTracker Dyes from Invitrogen can be used to make this differentiation. ABCB1运输者也用于区分过渡B细胞从天真B细胞。 染料例如Rhodamine123和MitoTracker染料从Invitrogen可以用于...