众所周知:在最初的抗原识别和激活后,naive B细胞可以在生发中心(GC)内经历连续几轮体细胞高频突变(SHM),最终导致同源抗原的高亲和力抗体。实验团队接下来就试图研究ab090等抗体如何通过SHM来提高亲和力和特异性,他们构建ab090和ab072随机突变库,得到了各位点的突变对亲和力的影响。后续通过假病毒中和实验证明了亲和力...
P Reichardt,B Dornbach,S Rong,S Beissert,M Gunzer 摘要: Naive B cells are ineffective antigen-presenting cells and are considered unable to activate naive T cells. However, antigen-specific contact of these cells leads to stable cell pairs that remain associated over hours in vivo. The ...
The perspective that naive B-cell recognition of antigen in the absence of T-cell help causes cell death or anergy is supported by in vivo studies of B cells that are continuously exposed to self-antigens. However, intravital imaging suggests that early
Endogenous antigen tunes the respon- siveness of naive B cells but not T cells. Nature 2012;489:160-4.Zikherman,J., Parameswaran,R., & Weiss,A. Endogenous antigen tunes the responsiveness of naive B cells but not T cells. Nature 489, 160- 164 (2012)....
Ighb strains had larger numbers of PE-specific naive B cells and generated smaller germinal center responses and larger numbers of IgM memory cells than the Igha strain. The properties of PE-specific B cells in Ighb mice correlated with usage of a single VH that afforded high-affinity PE ...
Such naive, idiotype-specific, CD4+T cells protected against tumor development of a class II negative plasmacytoma (MOPC315) and a class II positive B lymphoma (F9), which both secrete λ 2315immunoglobulin. Adoptive transfer experiments demonstrated that 2 X 106lymph node cells were sufficient ...
摘要: Objectives To examine the expression of B-cell-activating factor receptor (BAFF-R) on naive CD27− and memory CD27+ B cells in normal individuals and patients with rheumatoid arthritis (RA) undergoing B-cell depletion therapy with rituximab....
Tangye, Naive and memory B cells exhibit distinct biochemical responses following BCR engagement. Immunol Cell Biol, 2016. 94(8): p. 774-86.Moens L, Kane A, Tangye SG. Naive and memory B cells exhibit distinct biochemical responses follow- ing BCR engagement. Immunol Cell Biol. 2016; 94...
Neonatal B cells with the naive (sIgD + ) phenotype are able to generate IgG- and IgA-producing cells as well as IgM production in the presence of memory CD4 + T cells expressing L-selectin (CD62L) in pokeweed mitogen-stimulated cultures. We used this system to examine comparatively the ...
Transduction of naive B cells by lentiviral vectors was dependent on multiplicity of infection and did not lead to a concomitant activation. Furthermore, the transduced cells could be used for studies in the NOD mouse system without altering the onset of diabetes. We conclude that lentiviral gene...