Resistance to cisplatin-based chemotherapy limits the clinical benefit to some bladder cancer patients, and understanding the epigenetic regulation mechanism of cisplatin (CDDP) resistance in bladder cancer from the perspective of N6-methyladenosine (m6A) modification may optimize CDDP-based treatments. The...
近日,来自南方医科大学的研究者们在Molecular Cancer杂志上发表了题为“N6-methyladenosine-modified circPLPP4 sustains cisplatin resistance in ovarian cancer cells via PIK3R1 upregulation”的文章,该研究揭示了m6A诱导的circPLPP4/ miR-136/ PIK3R1轴介导OC中CDDP耐药的合理机制,表明circPLPP4可能作为CDDP耐药OC的...
The increased circPLPP4 level in CDDP-resistant cells was caused by increased RNA stability, mediated by increased N6-methyladenosine (m6A) modification of circPLPP4. In vivo delivery of an antisense oligonucleotide targeting circPLPP4 significantly enhanced CDDP efficacy in a tumor model. Conclusions...
FTO has also been reported to demethylate 5′ capN6,2′-O-dimethyladenosine (m6Am).71However, we found that internal m6A abundance is approximately 20–30 times of the near 5′ cap m6Amabundance in human AML cells as detected by liquid chromatography-tandem mass spectrometry (LC-MS/MS) a...
Internal N6-methyladenosine (m6A) modification is one of the most common and abundant modifications of RNA. However, the biological roles of viral RNA m6A remain elusive. Here, using human metapneumovirus (HMPV) as a model, we demonstrate that m6A serves as a molecular marker for innate immun...
2022年4月2日,来自中国中山大学肿瘤中心的Jin‑Ling Duan及其团队在Mol Cancer (IF: 15.302)杂志上发表名为A novel peptide encoded by N6-methyladenosine modified circMAP3K4 prevents apoptosis in hepatocellular carcinoma的研究[...
Breast cancer is the most common cancer type among women. Despite advanced treatment strategies, some patients still face challenges in disease control, prompting the exploration of new therapeutic approaches. N6-Methyladenosine (m6A) methylation modific
2. N6-methyladenosine 2.1. Methyltransferase The methyltransferase complex contains METTL3, METTL14 [14], regulatory subunit Wilms tumor suppressor-1-associated protein (WTAP) [15], and additional binding proteins, such as vir-like m6A methyltransferase associate protein (VIRMA), zinc finger CCCH doma...
N6-methyladenosine (m6A) is a prevalent internal modification in eukaryotic RNAs regulated by the so-called “writers”, “erasers”, and “readers”. m6A has been demonstrated to exert critical molecular functions in modulating RNA maturation, localizat
Here, we show that N6-methyladenosine (m6A) RNA modification on human circRNAs inhibits innate immunity. Foreign circRNAs are potent adjuvants to induce antigen-specific T cell activation, antibody production, and anti-tumor immunity in vivo, and m6A modification abrogates immune gene activation and ...