The scope and function of this modification in plants has been an intense focus of study, especially in model plant systems. The characterization of plant m6A writers, erasers and readers, as well as the elucidation of their functions, is currently one of the most fascinating hotspots in plant...
Proteins in gel slices or in the entire pulled-down protein sample were digested with trypsin and identified using LC–MS/MS by the Donald Danforth Plant Science Center. The gel-purified 5’32Plabeled RNA oligos were refolded by heating at 90oC for 1 min, then at 30oC for 5 min. A ...
Overall, our findings reveal that m6A-mediated sequestration of PYL7 by ECT8 in stress granules negatively regulates ABA perception, thereby enabling prompt feedback regulation of ABA signalling to prevent plant cell overreaction to environmental stresses....
As the most prevalent internal modification on eukaryotic mRNAs, N-methyladenosine (mA) exists in many plant species and requires the evolutionarily conserved methyltransferases, demethylases, and mA binding proteins for writing, erasing, and reading mA, respectively. In plants, mA affects many aspects...
Cell . MOLECULAR PLANT . 1 February 2023 Cell . Immunity . August 10, 2021 Nature Communications.1413 (2022) ACS . Nano Lett . April 10, 2023 加入购物车 N6-methyladenosine (m6A)详情 评价(0) 货号: ajci13638 CAS: 1867-73-8 别名: N6-甲基腺苷; 6-Methyladenosine; N-Methyla...
N6-methyladenosine (m6A) methylation, one of the most prevalent mRNA modifications, contributes to transcriptional reprogramming during plant responses to pathogen infection. Nevertheless, the role of m6A methylation in establishing compatible and incompatible soybean responses to SCN has not previously been...
(DMGs) and metabolites derived from theM. incognita-infected soybean roots. We found that there was a highly negative correlation between m6A methylation and the expression levels of some genes involved in the regulation of plant immune responses, including genes encoding transcription factors (TFs),...
Non-segmented negative-sense (NNS) RNA viruses encompass a wide range of significant human, animal, and plant pathogens. For many of these agents, there are no effective vaccines or antiviral drugs. The replication and gene expression strategy of NNS RNA viruses is unique. During transcription, ...
Previous studies characterized the m6A RNA methylomes of many RNA viruses, including human immunodeficiency virus, Zika virus, hepatitis C virus, enterovirus 71, murine leukemia virus, and the plant virus alfalfa mosaic virus (29, 30, 31, 32, 33, 34, 35, 36, 37, 38), as well as ...
Rice is one of the most important staple food and model species in plant biology, yet its quantitative proteomes are largely uncharacterized. Here we quantify the relative protein levels of over 15,000 genes across major rice tissues using a tandem mass