N-term 2017 was the first international meeting to bring together researchers from diverse disciplines with a shared interest in protein N-terminal modifications and the N-end rule pathway of ubiquitin-mediated proteolysis, providing a platform for interdisciplinary cross-kingdom discussions and ...
The key finding of this study is that the orphan GPCR, GPR37L1, is subject to N-terminal proteolysis by matrix metalloproteases both in cultured cells and rodent cerebellum, and that this post-translational modification is linked to loss of Gαs signal transduction. Using various biochemical and...
Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C...
Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, and chicken Shh-N. Shh-N can be palmitoylated at itsN-terminal cysteine...
(Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine, ...
Adenovirus composition, proteolysis, and disassembly studied by in-depth qualitative and quantitative proteomics 2014, Journal of Biological Chemistry Citation Excerpt : We found a similar behavior for VI, in which the amount of N-terminal fragment released was six times higher compared with the VI ...
an endoprotease complex that catalyzes the intramembrane cleavage of integral membrane proteins such as Notch receptors and APP (beta- amyloid precursor protein). Probably represents the last step of maturation of gamma-secretase, facilitating endoproteolysis of presenilin and conferring gamma-secretase act...
(Shh-N) and a glycosylated 25 kDa C-terminal protein (Shh-C) (4). Shh-C, which is responsible for the intramolecular processing of Shh, is rapidly degraded following Shh proteolysis (5). Shh-N is highly conserved, sharing >98% aa identity between mouse, human, rat, canine, porcine,...
It is known that the PhoA is assembled after translocation to the periplasm and becomes strongly resistant to proteolysis even in the presence of detergent [18]. The prePhoA is also resistant to proteolysis when translocated across the cytoplasmic membrane. In contrast, the prePhoA remaining in ...
Limited proteolysis of the PDEγ/Gαt cross-linked species with endo Lys-C selectively removed the Gαt N terminus. If PDEγ were cross-linked only to the Gαt N terminus, all PDEγ cross-links would have been removed concomitantly by limited digestion. In this case the trimmed Gαt-...