N-terminusprotein modificationproteostasisubiquitin proteasome systemN-term 2017 was the first international meeting to bring together researchers from diverse disciplines with a shared interest in protein N-terminal modifications and the N-end rule pathway of ubiquitin-mediated proteolysis, providing a ...
GPR37L1 is an orphan G protein-coupled receptor expressed exclusively in the brain and linked to seizures, neuroprotection and cardiovascular disease. Based upon the observation that fragments of the GPR37L1 N-terminus are found in human cerebrospinal fl
E, unique neo-N terminus (VVMD↓AcD3-568KSKGVPVISVKTSGSKER585) formed as in D but now generated exclusively through the action of human recombinant GrB in the scaffold attachment factor B protein. F, neo-N termini of the polypyrimidine tract-binding protein (generated at AAVD↓AcD3-173...
we assessed OAS-TL A turnover using the tandem-Fluorescent timer system, which allows for non-invasive quantification of relative protein half-lifetime in plants26. OAS-TL A’s relative half-lifetime with native N-terminus (ASROAS-TL) was significantly lower in NatA depleted plants ...
Thus, FtsH recognizes a cytosolic region of sufficient length (approximately 20 amino acids) to initiate the processive proteolysis against membrane proteins. Such a region is typically at the N-terminus and can be diverse in amino acid sequences. 展开 ...
Known N-recognins in eukaryotes mediate protein ubiquitylation and selective proteolysis by the 26S proteasome. Substrates of N-recognins can be generated when normally embedded destabilizing residues are exposed at the N terminus by proteolytic cleavage. N-degrons can also be generated through ...
1b). The fast-migrating H3 band is indicative of a chromatin-bound cleaved H3 (H3cl) product generated by proteolysis of the histone H3 N-terminus (H3NT) [14]. To corroborate these findings, the experiments were repeated using the rat L6 myoblast cell line, which undergo myoblast ...
Interaction of Psh1, an E3 ligase, with the C terminus of Cse4 has been shown to contribute to its proteolysis. Here, we demonstrate a role for ubiquitination of the N terminus of Cse4 in regulating Cse4 proteolysis for faithful chromosome segregation and a role for Doa1 in ubiquitination ...
Known N-recognins in eukaryotes mediate protein ubiquitylation andselective proteolysis by the 26S proteasome. Substrates of N-recognins can be generated whennormally embedded destabilizing residues are exposed at the N terminus by proteolytic cleavage.N-degrons can also be generated through ...
protein neddylation. Immunoblot analysis of neddylation pathway components using total cell extract (n = 3 biologically independent samples). All proteins have NatA-type N-termini, except CUL4B which has a NatC/E-type N-terminus (MM-). The upper cullin band represents the neddylated form ...