1323Accesses 10Altmetric Metrics Abstract ARGONAUTE (AGO) proteins bind to small non-coding RNAs to form RNA-induced silencing complexes. In the RNA-bound state, AGO is stable while RNA-free AGO turns over rapidly. Molecular features unique to RNA-free AGO that allow its specific recognition and...
3a). To exclude any effects at the transcriptional or translational levels, HAP1 WT and NAA30-KO cells were transfected with a bicistronic P2A reporter vector encoding UBE2M-V5-P2A-GST-GFP. Normalized UBE2M-V5 protein levels were significantly lower in NAA30-KO cells compared to WT cells ...
Adams.Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein[J]. Journal of Neural Transmission .2013(6)Cellular localization and trafficking of vascular adhesion protein-1 as revealed by an N-terminal GFP fusion protein[J] . Chris J...
MYC-GFP Mps1 WT rescued mitotic timing (43.78 ± 3.25 min), whereas Mps1-KD and Mps1-ΔNTE did not, with an average duration of 22.51 ± 0.97 min and 30.40 ± 1.25 min, respectively (p < 0.0001; ANOVA; Figure 6E). Although Mps1-Δ40–49-expressing cells completed mitosis at a ...
Transdermal absorption enhancement of n-terminal tat-GFP fusion protein(TG)loaded in novel low-toxic elastic anionic Niosomes[J].Journal of Pharmaceutical Sciences,2011,(04):1525-1534.Manosroi J, Lohcharoenkal W, Gotz F, Werner RG, Manosroi W, et al. (2011) Transdermal absorption enhancement...
Li T, Bourgeois JP, Celli S et al (2012) Cell-penetrating anti- GFAP VHH and corresponding fluorescent fusion protein VHH- GFP spontaneously cross the blood-brain barrier and specifically recognize astrocytes: application to brain imaging. FASEB J 26(10):3969–3979. doi:10.1096/fj.11-201384 ...
(C) Stability of tFT-tagged proteins in natBΔ relative to WT cells measured using the mCherry/GFP ratio as readout of imaging. Cells were grown under physiological (30°C) and heat stress (37°C for two doubling times [SWATH] or 1 day [tFT]) conditions. (D) Comparison of the stab...